Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures
	Chen, Shanshan 1; Rotaru, Amelia-Elena 1,2; Liu, Fanghua1,3 ; Philips, Jo 1; Woodard, Trevor L.1; Nevin, Kelly P.1; Lovley, Derek R.1
刊名	BIORESOURCE TECHNOLOGY
	2014-12-01
卷号	173 页码:82-86
关键词	Carbon cloth Syntrophy Direct interspecies electron transfer Geobacter Methanosarcina
ISSN号	0960-8524
通讯作者	Rotaru, AE (reprint author), Univ Southern Denmark, Nordic Ctr Earth Evolut, DK-5230 Odense S, Denmark. arotaru@biology.sdu.dk
产权排序	[Chen, Shanshan; Rotaru, Amelia-Elena; Liu, Fanghua; Philips, Jo; Woodard, Trevor L.; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Rotaru, Amelia-Elena] Univ Southern Denmark, Nordic Ctr Earth Evolut, DK-5230 Odense S, Denmark; [Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
中文摘要	This study investigated the possibility that the electrical conductivity of carbon cloth accelerates direct interspecies electron transfer (DIET) in co-cultures. Carbon cloth accelerated metabolism of DIET co-cultures (Geobacter metallireducens-Geobacter sulfurreducens and G. metallireducens-Methanosarcina barkeri) but did not promote metabolism of co-cultures performing interspecies H-2 transfer (Desulfovibrio vulgaris-G. sulfurreducens). On the other hand, DIET co-cultures were not stimulated by poorly conductive cotton cloth. Mutant strains lacking electrically conductive pili, or pili-associated cytochromes participated in DIET only in the presence of carbon cloth. In co-cultures promoted by carbon cloth, cells were primarily associated with the cloth although the syntrophic partners were too far apart for cell-to-cell biological electrical connections to be feasible. Carbon cloth seemingly mediated interspecies electron transfer between the distant syntrophic partners. These results suggest that the ability of carbon cloth to accelerate DIET should be considered in anaerobic digester designs that incorporate carbon cloth. (C) 2014 Elsevier Ltd. All rights reserved.
英文摘要	This study investigated the possibility that the electrical conductivity of carbon cloth accelerates direct interspecies electron transfer (DIET) in co-cultures. Carbon cloth accelerated metabolism of DIET co-cultures (Geobacter metallireducens-Geobacter sulfurreducens and G. metallireducens-Methanosarcina barkeri) but did not promote metabolism of co-cultures performing interspecies H-2 transfer (Desulfovibrio vulgaris-G. sulfurreducens). On the other hand, DIET co-cultures were not stimulated by poorly conductive cotton cloth. Mutant strains lacking electrically conductive pili, or pili-associated cytochromes participated in DIET only in the presence of carbon cloth. In co-cultures promoted by carbon cloth, cells were primarily associated with the cloth although the syntrophic partners were too far apart for cell-to-cell biological electrical connections to be feasible. Carbon cloth seemingly mediated interspecies electron transfer between the distant syntrophic partners. These results suggest that the ability of carbon cloth to accelerate DIET should be considered in anaerobic digester designs that incorporate carbon cloth. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	PACKED-BED REACTOR ; GEOBACTER-SULFURREDUCENS ; MICROBIAL-POPULATION ; ANAEROBIC-DIGESTION ; SOLID-WASTE ; SP-NOV ; REDUCTION ; METHANOSAETA ; HYDROGEN ; GROWTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000344120200011
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8523]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA 2.Univ Southern Denmark, Nordic Ctr Earth Evolut, DK-5230 Odense S, Denmark 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Chen, Shanshan,Rotaru, Amelia-Elena,Liu, Fanghua,et al. Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures[J]. BIORESOURCE TECHNOLOGY,2014,173:82-86.
APA	Chen, Shanshan.,Rotaru, Amelia-Elena.,Liu, Fanghua.,Philips, Jo.,Woodard, Trevor L..,...&Lovley, Derek R..(2014).Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures.BIORESOURCE TECHNOLOGY,173,82-86.
MLA	Chen, Shanshan,et al."Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures".BIORESOURCE TECHNOLOGY 173(2014):82-86.