Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient
	Ma, B. ; Lyu, X. -F. ; Zha, T. ; Gong, J. ; He, Y. ; Xu, J. -M.
刊名	JOURNAL OF APPLIED MICROBIOLOGY
	2015-04
卷号	118 期号:4 页码:890-900
关键词	microbial community phytoremediation polycyclic aromatic hydrocarbons reconstructed metagenome rhizosphere
ISSN号	1364-5072
通讯作者	Xu, JM (reprint author), Zhejiang Univ, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Inst Soil & Water Resources & Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China. jmxu@zju.edu.cn
产权排序	[Ma, B.; Zha, T.; He, Y.; Xu, J. -M.] Zhejiang Univ, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Inst Soil & Water Resources & Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China; [Ma, B.; Lyu, X. -F.; Gong, J.] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Microbial Ecol & Matter Cycles, Yantai, Peoples R China
中文摘要	AimsThe response of microbial metagenome to polycyclic aromatic hydrocarbons (PAHs) degradation in the rice rhizosphere remains poorly understood. We investigated the spatial and temporal variations of microbial communities and reconstructed metagenomes along the rice rhizosphere gradient during PAHs degradation. Methods and ResultsThe experiment was performed in rhizoboxes, in which the rhizosphere region was divided into five 1-mm thick layers. Based on denaturant gradient gel electrophoresis profiling and sequencing of bacterial and archaeal 16S rRNA genes, predicted metagenomes were reconstructed. The microbial communities in the rice rhizosphere were influenced by the PAHs concentration and distance from the root surface during PAHs degradation. Correlation network analysis showed that archaea played an important role in PAHs degradation. Predicted metagenomes can be clustered into two groups with high and low PAHs degrading potential, respectively. The relative abundance of genes for defense mechanisms, replication, recombination and reparation was significantly higher in samples with high PAHs degrading potentials. The relative abundance of the dioxygenase gene was greater near the root surface of the rice. However, the abundance of aldolase and dehydrogenase was constant in rhizosphere soils at different distances from the root surface. ConclusionsDistance from root surface and PAH concentrations affected the microbial communities and metagenomes in rice rhizosphere. The abundance of dioxygenase genes relating to PAH degradation in metagenomes mirrored the PAH degradation potential in rice rhizosphere. Significance and Impact of the StudyOur findings suggested that the predicted metagenomes reconstructed from 16S rRNA marker gene sequences provide further insights into the spatial variation and dynamics of microbial functioning that occur during bioremediation.
学科主题	Biotechnology & Applied Microbiology; Microbiology
研究领域[WOS]	National Natural Science Foundation of China [41090284, 41301333]
关键词[WOS]	POLYCYCLIC AROMATIC-HYDROCARBONS ; ANAEROBIC DEGRADATION ; BACTERIAL COMMUNITIES ; GENE-SEQUENCES ; SOIL ; BIODEGRADATION ; PENTACHLOROPHENOL ; DIVERSITY ; SEDIMENT ; IMPACT
收录类别	SCI
语种	英语
WOS记录号	WOS:000351392200012
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8507]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式 GB/T 7714	Ma, B.,Lyu, X. -F.,Zha, T.,et al. Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient[J]. JOURNAL OF APPLIED MICROBIOLOGY,2015,118(4):890-900.
APA	Ma, B.,Lyu, X. -F.,Zha, T.,Gong, J.,He, Y.,&Xu, J. -M..(2015).Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient.JOURNAL OF APPLIED MICROBIOLOGY,118(4),890-900.
MLA	Ma, B.,et al."Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient".JOURNAL OF APPLIED MICROBIOLOGY 118.4(2015):890-900.