Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)

	Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)
	Que, H ; Allen, SK
刊名	JOURNAL OF SHELLFISH RESEARCH
	2002-06-01
卷号	21 期号:1 页码:137-143
关键词	Crassostrea gigas Crassostrea ariakensis diploid hybrid tetraploid oyster breeding polyploidy
英文摘要	Three replicates of hybrid crosses of tetraploid and diploid C gigas (Thunberg) with diploid C ariakensis (Fujita) were produced with controls. Larval survival and growth were documented. Cytological events were also monitored in oocytes from hybrid crosses following insemination. Among the four types of hybrid crosses, diploid C. gigas (female) x diploid C. ariakensis (male) (GA) was the most successful. Survival of GA was about the same as that of controls in two of three replications, although its growth rate was 25-30% lower. Crosses of tetraploid C. gigas (female) and diploid C. ariakensis (male) (GGA) had poor yield at day 2 post-fertilization (0.05%), but grew nearly as well as controls subsequently. The other two types of hybrids (i.e., diploid C. ariakensis [female] and tetraploid C. gigas [male] [AGG], diploid C ariakensis [female] and diploid C. gigos [male] [AG]) suffered very low yield at day 2 (0.01% and 0.003%) and grew very slowly. Spat were obtained from all replicates of GA crosses and one of three replicates of GGA, and proved to be hybrids by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) diagnosis. GGA hybrids were confirmed to be triploid by flow cytometry. No larvae survived to eyed stage in AGG or AG crosses. Cytological examination revealed that the vast majority (>99%) of oocytes from hybrid crosses had a prolonged meiotic prophase I or metaphase I at least through 180 min post-insemination.
收录类别	SCI
语种	英语
WOS记录号	WOS:000176764900020
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/32992]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
推荐引用方式 GB/T 7714	Que, H,Allen, SK. Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)[J]. JOURNAL OF SHELLFISH RESEARCH,2002,21(1):137-143.
APA	Que, H,&Allen, SK.(2002).Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita).JOURNAL OF SHELLFISH RESEARCH,21(1),137-143.
MLA	Que, H,et al."Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)".JOURNAL OF SHELLFISH RESEARCH 21.1(2002):137-143.