Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research
	Wang, Guifang 1; Zhang, Ze-Ting 2,3; Jiang, Bin3 ; Zhang, Xu 3; Li, Conggang 2,3; Liu, Maili 3
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2014-04-01
卷号	406 期号:9-10 页码:2279-2288
关键词	Protein NMR Protein therapeutics Posttranslational modifications Protein aggregation
英文摘要	Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography are the two main methods for protein three-dimensional structure determination at atomic resolution. According to the protein structures deposited in the Protein Data Bank, X-ray crystallography has become the dominant method for structure determination, particularly for large proteins and complexes. However, with the developments of isotope labeling, increase of magnetic field strength, common use of a cryogenic probe, and ingenious pulse sequence design, the applications of NMR spectroscopy have expanded in biological research, especially in characterizing protein dynamics, sparsely populated transient structures, weak protein interactions, and proteins in living cells at atomic resolution, which is difficult if not impossible by other biophysical methods. Although great advances have been made in protein NMR spectroscopy, its applications in protein therapeutics, which represents the fastest growing segment of the pharmaceutical industry, are still limited. Here we review the recent advances in the use of NMR spectroscopy in studies of large proteins or complexes, posttranslation modifications, weak interactions, and aggregation, and in-cell NMR spectroscopy. The potential applications of NMR spectroscopy in protein therapeutic assays are discussed.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	NUCLEAR-MAGNETIC-RESONANCE ; HIGH-RESOLUTION NMR ; PARAMAGNETIC RELAXATION ENHANCEMENT ; IN-CELL ; LIVING CELLS ; ESCHERICHIA-COLI ; STATE NMR ; F-19 NMR ; DYNAMICS ; BIOPHARMACEUTICALS
收录类别	SCI
语种	英语
WOS记录号	WOS:000334171200005
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1321]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Wuhan Branch, Natl Sci Lib, Wuhan Documentat & Informat Ctr, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Key Lab Magnet Resonance Biol Syst, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Guifang,Zhang, Ze-Ting,Jiang, Bin,et al. Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(9-10):2279-2288.
APA	Wang, Guifang,Zhang, Ze-Ting,Jiang, Bin,Zhang, Xu,Li, Conggang,&Liu, Maili.(2014).Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,406(9-10),2279-2288.
MLA	Wang, Guifang,et al."Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.9-10(2014):2279-2288.