Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States
	Lan, Wenxian 1; Wang, Zhonghua 2; Yang, Zhongzheng 1; Zhu, Jing 1; Ying, Tianlei 2; Jiang, Xianwang 3; Zhang, Xu 3; Wu, Houming 1; Liu, Maili 3; Tan, Xiangshi 2
刊名	PLOS ONE
	2011-11-08
卷号	6 期号:11 页码:e27219-e27219
产权排序	第一
英文摘要	To convert cyt c into a peroxidase-like metalloenzyme, the P71H mutant was designed to introduce a distal histidine. Unexpectedly, its peroxidase activity was found even lower than that of the native, and that the axial ligation of heme iron was changed to His71/His18 in the oxidized state, while to Met80/His18 in the reduced state, characterized by UV-visible, circular dichroism, and resonance Raman spectroscopy. To further probe the functional importance of Pro71 in oxidation state dependent conformational changes occurred in cyt c, the solution structures of P71H mutant in both oxidation states were determined. The structures indicate that the half molecule of cyt c (aa 50-102) presents a kind of "zigzag riveting ruler" structure, residues at certain positions of this region such as Pro71, Lys73 can move a big distance by altering the tertiary structure while maintaining the secondary structures. This finding provides a molecular insight into conformational toggling in different oxidation states of cyt c that is principle significance to its biological functions in electron transfer and apoptosis. Structural analysis also reveals that Pro71 functions as a key hydrophobic patch in the folding of the polypeptide of the region (aa 50-102), to prevent heme pocket from the solvent.
学科主题	波谱学
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	TRANSCRIPTIONAL ACTIVATOR COOA ; MITOCHONDRIAL CYTOCHROME-C ; GATED ELECTRON-TRANSFER ; CIRCULAR-DICHROISM ; FERRICYTOCHROME-C ; LIGAND-EXCHANGE ; MET80X MUTANTS ; ALKALINE FORM ; AXIAL LIGAND ; HEME
收录类别	SCI
语种	英语
WOS记录号	WOS:000297349700020
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1897]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Fudan Univ, Dept Chem, Biol Chem Lab, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Lan, Wenxian,Wang, Zhonghua,Yang, Zhongzheng,et al. Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States[J]. PLOS ONE,2011,6(11):e27219-e27219.
APA	Lan, Wenxian.,Wang, Zhonghua.,Yang, Zhongzheng.,Zhu, Jing.,Ying, Tianlei.,...&Huang, Zhong-Xian.(2011).Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States.PLOS ONE,6(11),e27219-e27219.
MLA	Lan, Wenxian,et al."Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States".PLOS ONE 6.11(2011):e27219-e27219.