Dynamic Metabolic Response of Mice to Acute Mequindox Exposure

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Dynamic Metabolic Response of Mice to Acute Mequindox Exposure
	Zhao, Xiu-Ju 1,2; Huang, Chongyang 1,2; Lei, Hehua 1; Nie, Xiu 3; Tang, Huiru 1; Wang, Yulan 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2011-11-01
卷号	10 期号:11 页码:5183-5190
关键词	NMR pattern recognition metabonomics mequindox dose effect time effect mice
产权排序	第一
英文摘要	Mequindox is used as a veterinary antibiotic drug. As part of systematic investigations into mequindox as a veterinary medicine and its subsequent applications in food safety, we conducted the investigation to assess the metabolic response of mice to mequindox using metabonomics, which combines NMR metabolic profiles of biofluids or tissues and pattern recognition data analysis. In this study, we delivered a single dose of mequindox to mice with dosage levels of 15, 75, and 350 mg/kg body weight and collected urine samples over a 7 day period, as well as plasma and liver tissues at 7 days postdose. Principal components analysis (PCA) and orthogonal projection to latent structure discriminant analysis (O-PLS-DA) were performed on (1)H NMR spectra of biofluids and liver, showing that low dose levels of mequindox exposure had no adverse effects, consistent with histological observations of the liver. High and moderate levels of mequindox exposure caused suppression of glycolysis and stimulation of fatty acid oxidation accompanied with increased levels of oxidative stress. Our metabonomic analyses also showed disruption of amino acid metabolism, consistent with liver damage observed from histopathological examinations. Furthermore, mequindox perturbed gut microbial activity manifested in the altered excretion of urinary trimethylamine (TMA), trimethylamine-N-oxide (TMAO), hippurate, phenylacetylglycine (PAG), and phenylacetate. The putative gut microbial function may also contribute to the assembly and secretion of very-low-density lipoproteins from the liver to the plasma. Our work provides important insights on the metabolic responses of mequindox.
学科主题	波谱学
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	PRINCIPAL COMPONENT ANALYSIS ; MATRICARIA-RECUTITA L. ; LEAST-SQUARES METHODS ; METABONOMIC INVESTIGATIONS ; MAGNETIC-RESONANCE ; SPECTRAL ANALYSES ; MODEL ; RATS ; PLS ; CLASSIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000296414700026
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1892]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Huazhong Univ Sci & Technol, Dept Pathol, Union Hosp, Tongji Med Coll, Wuhan 430022, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xiu-Ju,Huang, Chongyang,Lei, Hehua,et al. Dynamic Metabolic Response of Mice to Acute Mequindox Exposure[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(11):5183-5190.
APA	Zhao, Xiu-Ju,Huang, Chongyang,Lei, Hehua,Nie, Xiu,Tang, Huiru,&Wang, Yulan.(2011).Dynamic Metabolic Response of Mice to Acute Mequindox Exposure.JOURNAL OF PROTEOME RESEARCH,10(11),5183-5190.
MLA	Zhao, Xiu-Ju,et al."Dynamic Metabolic Response of Mice to Acute Mequindox Exposure".JOURNAL OF PROTEOME RESEARCH 10.11(2011):5183-5190.