NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism
	Feng, Jianghua 1; Zhao, Jing 2; Hao, Fuhua1 ; Chen, Chang 2; Bhakoo, Kishore 3; Tang, Huiru 1
刊名	JOURNAL OF NANOPARTICLE RESEARCH
	2011-05-01
卷号	13 期号:5 页码:2049-2062
关键词	Metabonomics USPIO NMR spectroscopy Macrophage Nanobiology Nanomedicine
通讯作者	唐惠儒
产权排序	第一
英文摘要	The metabonomic changes in murine RAW264.7 macrophage-like cell line induced by ultrasmall superparamagnetic particles of iron oxides (USPIO) have been investigated, by analyzing both the cells and culture media, using high-resolution NMR in conjunction with multivariate statistical methods. Upon treatment with USPIO, macrophage cells showed a significant decrease in the levels of triglycerides, essential amino acids such as valine, isoleucine, and choline metabolites together with an increase of glycerophospholipids, tyrosine, phenylalanine, lysine, glycine, and glutamate. Such cellular responses to USPIO were also detectable in compositional changes of cell media, showing an obvious depletion of the primary nutrition molecules, such as glucose and amino acids and the production of end-products of glycolysis, such as pyruvate, acetate, and lactate and intermediates of TCA cycle such as succinate and citrate. At 48 h treatment, there was a differential response to incubation with USPIO in both cell metabonome and medium components, indicating that USPIO are phagocytosed and released by macrophages. Furthermore, information on cell membrane modification can be derived from the changes in choline-like metabolites. These results not only suggest that NMR-based metabonomic methods have sufficient sensitivity to identify the metabolic consequences of murine RAW264.7 macrophage-like cell line response to USPIO in vitro, but also provide useful information on the effects of USPIO on cellular metabolism.
学科主题	波谱分析
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science
关键词[WOS]	SPINNING H-1-NMR SPECTROSCOPY ; MAGNETIC-RESONANCE-SPECTROSCOPY ; MULTIVARIATE DATA-ANALYSIS ; HIGH-RESOLUTION H-1 ; MR CONTRAST AGENTS ; IN-VITRO ; SCHISTOSOMA-MANSONI ; CELLS ; NANOPARTICLES ; RAT
收录类别	SCI
语种	英语
WOS记录号	WOS:000290577600023
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1788]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 3.ASTAR, Singapore Bioimaging Consortium, Singapore, Singapore
推荐引用方式 GB/T 7714	Feng, Jianghua,Zhao, Jing,Hao, Fuhua,et al. NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism[J]. JOURNAL OF NANOPARTICLE RESEARCH,2011,13(5):2049-2062.
APA	Feng, Jianghua,Zhao, Jing,Hao, Fuhua,Chen, Chang,Bhakoo, Kishore,&Tang, Huiru.(2011).NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism.JOURNAL OF NANOPARTICLE RESEARCH,13(5),2049-2062.
MLA	Feng, Jianghua,et al."NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism".JOURNAL OF NANOPARTICLE RESEARCH 13.5(2011):2049-2062.