In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR
	Fu, Riqiang 2; Wang, Xingsheng 1; Li, Conggang 3; Santiago-Miranda, Adriana N.4; Pielak, Gary J.5; Tian, Fang 1
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2011-08-17
卷号	133 期号:32 页码:12370-12373
通讯作者	李从刚
产权排序	第三
英文摘要	The feasibility of using solid-state magic-angle-spinning NMR spectroscopy for in situ structural characterization of the LR11 (sorLA) transmembrane domain (TM) in native Escherichia coli membranes is presented. LR11 interacts with the human amyloid precursor protein (APP), a central player in the pathology of Alzheimer's disease. The background signals from E. coli lipids and membrane proteins had only minor effects on the LR11 TM resonances. Approximately 50% of the LR11 TM residues were assigned by using C-13 PARIS data. These assignments allowed comparisons of the secondary structure of the LR11 TM in native membrane environments and commonly used membrane mimics (e.g., micelles). In situ spectroscopy bypasses several obstacles in the preparation of membrane proteins for structural analysis and offers the opportunity to investigate how membrane heterogeneity, bilayer asymmetry, chemical gradients, and macromolecular crowding affect the protein structure.
学科主题	无线电物理
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	AMYLOID PRECURSOR PROTEIN ; RESONANCE ASSIGNMENT ; ALZHEIMER-DISEASE ; SECONDARY STRUCTURE ; PURPLE MEMBRANES ; LIPID-MEMBRANES ; COAT PROTEIN ; C-13 ; BACTERIORHODOPSIN ; SPECTROSCOPY
收录类别	SCI
语种	英语
WOS记录号	WOS:000294740000014
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1752]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Penn State Univ, Dept Biochem & Mol Biol, Hershey, PA 17033 USA 2.Natl High Magnet Field Lab, Tallahassee, FL 32310 USA 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 4.Univ Puerto Rico, Dept Chem Engn, Mayaguez, PR 00681 USA 5.Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA
推荐引用方式 GB/T 7714	Fu, Riqiang,Wang, Xingsheng,Li, Conggang,et al. In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2011,133(32):12370-12373.
APA	Fu, Riqiang,Wang, Xingsheng,Li, Conggang,Santiago-Miranda, Adriana N.,Pielak, Gary J.,&Tian, Fang.(2011).In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,133(32),12370-12373.
MLA	Fu, Riqiang,et al."In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 133.32(2011):12370-12373.