The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas

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	The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas
	Liu, Zhaoqun1,2 ; Zhou, Zhi 1; Wang, Lingling 1; Jiang, Shuai 1; Wang, Weilin1,2 ; Zhang, Ran 1,3; Song, Linsheng 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2015-04-01
卷号	49 期号:2 页码:217-224
关键词	Crassostrea gigas delta-opioid receptor [Met(5)]-enkephalin Immunomodulation Haemocyte
英文摘要	Opioid receptors (OR) are a group of G protein-coupled receptors with opioids as ligands, which play an important role in triggering the second messengers to modulate immune response in vertebrate immunocytes. In the present study, the full length cDNA of a homologue of delta-opioid receptor (DOR) for [Met(5)]-enkaphalin was cloned from oyster Crassostrea gigas (designated as CgDOR), which was 1104 bp encoding a peptide of 367 amino acids containing a conserved 7tm_1 domain. After the stimulation of [Met(5)]-enkephalin, the concentration of second messengers Ca2+ and cAMP in the HEK293T cells decreased significantly (p < 0.05) with the expression of CgDOR. However, this trend was reverted with the addition of DOR antagonist BNTX. The CgDOR transcripts were ubiquitously detected in the tested tissues including haemocytes, gonad, mantle, kidney, gill, adductor muscle and hepatopancreas, with the highest expression level in the hepatopancreas. After LPS stimulation, the expression level of CgDOR mRNA began to increase (4.05-fold, p < 0.05) at 6 h, and reached the highest level (5.00-fold, p < 0.05) at 12 h. Haemocyte phagocytic and antibacterial activities increased significantly after [Met(5)]-enkephalin stimulation, whereas the increase was repressed with the addition of DOR antagonist BNTX. These results collectively suggested that CgDOR for [Met(5)]-enkephalin could modulate the haemocyte phagocytic and antibacterial functions through the second messengers Ca2+ and cAMP, which might be requisite for pathogen elimination and homeostasis maintenance in oyster. (C) 2014 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000349880500002
公开日期	2015-06-16
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/25370]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,49(2):217-224.
APA	Liu, Zhaoqun.,Zhou, Zhi.,Wang, Lingling.,Jiang, Shuai.,Wang, Weilin.,...&Song, Linsheng.(2015).The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,49(2),217-224.
MLA	Liu, Zhaoqun,et al."The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 49.2(2015):217-224.