Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino

	Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino
	Ding, Jianfeng 2; Li, Li 1; Zhang, Guofan 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-02-01
卷号	42 期号:2 页码:379-383
关键词	Haliotis discus hannai Cysteine protease inhibitor CPI Immunity
英文摘要	The sequence of the cysteine protease inhibitor gene of Haliotis discus hannai (designated HdCpi) was determined using the RACE method. The full-length HdCpi cDNA is 1049 bp long, and contains an open reading frame of 813 bp, encoding a 271-amino-acid protein with a calculated molecular mass of 29.83 kDa and an isoelectric point of 8.57. The deduced amino acid sequence of HdCpi contains two cystatin-like domains, and each has the structural features of the cystatin family, including three evolutionarily conserved motifs known to interact with the active sites of cysteine peptidases: the Gly residue at the N-terminus (Gly(65) and Gly(160)), the Gln-X-Val-X-Gly motif (Q(106)IVSO(110) and Q(202)VVAG(206),) and the less conserved motif at the C-terminus ((SW137)-W-136 and A(254)W(255)). Many putative transcription-factor-binding sites involved in the immune system and cancer occur in the promoter region of HdCpi. Quantitative real-time RT-PCR detected HdCpi expression in all the tissues examined and in the gills of abalone challenged with the bacterium Vibrio anguillarum. HdCpi transcripts were expressed in the mantle, gill, digestive tract, hemocytes, and muscle, and increased HdCpi expression was observed after bacterial stimulation. These results suggest that HdCpi is a biologically active protease inhibitor that is likely to be involved in the antibacterial response of the abalone. (C) 2014 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000348621700020
公开日期	2015-06-16
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/25366]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jianfeng,Li, Li,Zhang, Guofan. Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):379-383.
APA	Ding, Jianfeng,Li, Li,&Zhang, Guofan.(2015).Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino.FISH & SHELLFISH IMMUNOLOGY,42(2),379-383.
MLA	Ding, Jianfeng,et al."Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):379-383.