Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii
	Li, Y.
刊名	Planta
	2001
卷号	213 期号:5 页码:722-730
ISSN号	0032-0935
中文摘要	The distribution of and relationship between F-actin and G-actin were investigated in pollen grains and pollen tubes of Lilium davidii Duch. using a confocal laser scanning microscope after fluorescence and immunofluorescence labeling. Circular F-actin bundles were found to be the main form of microfilament cytoskeleton in pollen grains and pollen tubes. Consistent with cytoplasmic streaming in pollen tubes, there were no obvious F-actin bundles in the 10- to 20-mum tip region of long pollen tubes, only a few short F-actin fragments. Labeling with fluorescein isothiocyanate (FITC)-DNase I at first established the presence of a tip-focused gradient of intracellular G-actin concentration at the extreme apex of the tube, the concentration of G-actin being about twice as high in the 10- to 20-mum region of the tip as in other regions of the pollen tube. We also found that the distribution of G-actin was related negatively to that of the F-actin in pollen tubes of L. davidii. Caffeine treatment caused the G-actin tip-focused gradient to disappear, and F-actin to extend into the pollen tube tip. Based on these results, we speculate that the circular F-actin bundles may be the track for bidirectional cytoplasmic streaming in pollen tubes, and that in the pollen tube tip most of the F-actin is depolymerized into G-actin, leading to the absence of F-actin bundles in this region.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8975]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Li, Y.. Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii[J]. Planta,2001,213(5):722-730.
APA	Li, Y..(2001).Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii.Planta,213(5),722-730.
MLA	Li, Y.."Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii".Planta 213.5(2001):722-730.