Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation

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	Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation
	Li, Y. X.
刊名	Cellular Physiology and Biochemistry
	2014
卷号	33 期号:4 页码:1186-1196
ISSN号	1015-8987
中文摘要	Background: Sonodynamic therapy (SDT) is an alternative strategy that manages malignancies via the generation of cytotoxic factors during ultrasound-activated sono-sensitive agents. However, the detailed mechanisms are not clear. This study was to identify the cytotoxic effects of ultrasound-activated protoporphyrin IX (PpIX) on U937 cells. Methods: Flow cytometry was performed to detect the time course for PpIX uptake in U937 cells. Sub-cellular localization of PpIX in U937 cells was visualized by inverted confocal laser scanning microscope. Following PpIX-mediated SDT treatment, cell viability was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay; nuclear damage was observed under fluorescent microscope; DNA fragmentation and mitochondrial membrane potential disruption were measured by flow cytometry. The role of reactive oxygen species (ROS) in SDT-induced cell death was also evaluated. Results: We observed that PpIX is mainly localized in the mitochondria, with a maximal uptake within 2 h. Compared with PpIX or ultrasound alone, PpIX plus ultrasound treatment significantly declined cell viability, caused more serious damage of cell morphology, DNA and mitochondria. In the combined treatment group, the intracellular ROS was greatly higher than in other groups; ROS scavenger N-acetylcysteine could effectively rescue the loss of mitochondria membrane potential and cell viability induced by SDT. Conclusion: Taken together, these findings primarily indicated that fatal damage could be induced by PpIX-mediated SDT in U937 cells, and the intracellular ROS was involved during this process. Copyright (C) 2014 S. Karger AG, Basel
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8938]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Li, Y. X.. Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation[J]. Cellular Physiology and Biochemistry,2014,33(4):1186-1196.
APA	Li, Y. X..(2014).Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation.Cellular Physiology and Biochemistry,33(4),1186-1196.
MLA	Li, Y. X.."Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation".Cellular Physiology and Biochemistry 33.4(2014):1186-1196.