Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells

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	Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells
	Masuda-Murakami, Y.
刊名	Acta Histochemica
	2010
卷号	112 期号:5 页码:452-458
ISSN号	0065-1281
中文摘要	The effect of mineral trioxide aggregate (MTA) on the odontoblast-like differentiation of pulp cells was evaluated using heat-shock protein 25 (hsp25) as a marker for odontoblast differentiation. The cells were cultured with tooth-colored MTA or calcium hydroxide-containing cement (Dycal). The effects of the materials on the pulp cells were observed using a confocal laser scanning microscope. The cells were labelled immunocytochemically using polyclonal antibodies against hsp25 and actin. The mRNA expression of hsp25 and dspp in the pulp cells at 2 days were examined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Most of the cells cultured with MTA showed an intense immunlabelling for hsp25 and the mRNA expressions of hsp25 and dspp at 2 days were higher than those cultured with Dycal. These findings indicate that MTA is an effective pulp capping material and is able to induce the differentiation of odontoblast-like cells and the formation of reparative tertiary dentin with minimum apoptosis. (C) 2009 Elsevier GmbH. All rights reserved.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8913]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Masuda-Murakami, Y.. Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells[J]. Acta Histochemica,2010,112(5):452-458.
APA	Masuda-Murakami, Y..(2010).Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells.Acta Histochemica,112(5),452-458.
MLA	Masuda-Murakami, Y.."Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells".Acta Histochemica 112.5(2010):452-458.