HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo a pyrene and interacts with casein kinase 2

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo a pyrene and interacts with casein kinase 2
	Duan, Y. Y.
刊名	Cell Stress & Chaperones
	2014
卷号	19 期号:2 页码:271-279
ISSN号	1355-8145
中文摘要	Benzo[a]pyrene (BaP) is a ubiquitously distributed environmental pollutant that induces deoxyribonucleic acid (DNA) damage. The inducible heat shock protein (HspA1A) can function as a molecular chaperone; however, its role in DNA repair remains largely unknown. In the present study, human bronchial epithelial cells (16HBE) stably transfected with plasmids carrying HspA1A gene or shRNAs against HspA1A were treated with BaP. DNA damage levels of the cells were evaluated by comet assay. Results suggest that HspA1A could protect cells against DNA damage and facilitate the decrease of DNA damage levels during the first 2 h of DNA repair. DNA repair capacity (DRC) of Benzo(a)pyrene diol epoxide (BPDE)-DNA adducts was evaluated by host cell reactivation assay in the stable 16HBE cells transfected with luciferase reporter vector PCMVluc pretreated with BPDE. Compared with control cells, cells overexpressing HspA1A showed higher DRC (p < 0.01 at 10 mu M BPDE and p < 0.05 at 20 mu M BPDE, respectively), while knockdown of HspA1A inhibited DNA repair (p < 0.05 at 10 mu M BPDE). Moreover, casein kinase 2 (CK2) was shown to interact with HspA1A by mass spectrometry and co-immunoprecipitation assays. The two proteins were co-localized in the cell nucleus and perinuclear region during DNA repair, and were identified by confocal laser scanning microscope. In addition, cells overexpressing HspA1A showed an increased CK2 activity after BaP treatment compared with control cells (p < 0.01). Our results suggest that HspA1A facilitates DNA repair after BaP treatment. HspA1A also interacts with CK2 and enhances the kinase activities of CK2 during DNA repair.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8855]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Duan, Y. Y.. HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo a pyrene and interacts with casein kinase 2[J]. Cell Stress & Chaperones,2014,19(2):271-279.
APA	Duan, Y. Y..(2014).HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo a pyrene and interacts with casein kinase 2.Cell Stress & Chaperones,19(2),271-279.
MLA	Duan, Y. Y.."HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo a pyrene and interacts with casein kinase 2".Cell Stress & Chaperones 19.2(2014):271-279.