The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon host cell interaction

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	The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon host cell interaction
	de-Souza, W.
刊名	Brazilian Journal of Medical and Biological Research
	1998
卷号	31 期号:11 页码:1459-1470
ISSN号	0100-879X
中文摘要	In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid (FITC-thiosemicarbazide) before interaction with the parasites. Evidence was obtained indicating transfer of components of the host cell surface to the parasite surface in the beginning of the interaction process. We also analyzed the distribution of cytoskeletal structures (microtubules and microfilaments visualized with specific antibodies), mitochondria (visualized with rhodamine 123), the Golgi complex (visualized with C6-NBD-ceramide) and the endoplasmic reticulum (visualized with anti-reticulin antibodies and DIOC6) during the evolution of intracellular parasitism. The results obtained show that some, but not all, structures change their position during evolution of the intracellular parasitism.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8771]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	de-Souza, W.. The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon host cell interaction[J]. Brazilian Journal of Medical and Biological Research,1998,31(11):1459-1470.
APA	de-Souza, W..(1998).The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon host cell interaction.Brazilian Journal of Medical and Biological Research,31(11),1459-1470.
MLA	de-Souza, W.."The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon host cell interaction".Brazilian Journal of Medical and Biological Research 31.11(1998):1459-1470.