Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae)

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	Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae)
	Deng, Yunyan 1; Yao, Jianting 1; Fu, Gang 1; Guo, Hui 2; Duan, Delin 1
刊名	MARINE BIOTECHNOLOGY
	2014-04-01
卷号	16 期号:2 页码:135-143
关键词	Aureochrome Blue light receptor bZIP domain LOV domain Saccharina japonica Stramenopile
通讯作者	Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	Photosynthetic stramenopile have chloroplasts of secondary endosymbiotic origin and are significant as aquatic primary productivity and biomass production. In marine environments, many photosynthetic stramenopiles utilize blue light to regulate growth, development, and organelle movement. Aureochrome (AUREO) is a new type blue light photoreceptor specific in photosynthetic stramenopiles. Previously, several AUREO orthologs were reported in genomes of stramenopile members, but the full-length cDNA sequences were completed only in Vaucheria frigida (Xanthophyceae), Fucus distichus (Phaeophyceae), and Ochromonas danica (Chrysophyceae). In this study, the full-length cDNA of AUREO from Saccharina japonica (designated as SjAUREO) was isolated based on homologous cloning and the rapid amplification of cDNA ends (RACE). It characterized by the full length of 1,013 bp with an open reading frame of 612 bp, which encoded a polypeptide of 203 amino acids with predicted molecular weight of 23.08 kDa and theoretical isoelectric point of 7.63. The deduced amino acid sequence of SjAUREO contained one N-terminal basic region/leucine zipper (bZIP) transcription regulation domain and a single light-, oxygen-, or voltage-sensitive (LOV) domain near the C-terminus. Homologous analysis showed that SjAUREO shared 40-92 % similarities with those of other photosynthetic stramenopiles. Phylogenetic analysis revealed close phylogenetic affinity between SjAUREO and AUREO4 of brown alga Ectocarpus siliculosus. Real-time PCR detection revealed that the SjAUREO transcription was markedly increased under BL exposure and dramatically upregulated in the 1-month juvenile sporophyte than those in the 2 and 3-month materials, which indirectly reflected the SjAUREO associated with the BL-mediated photomorphogenesis during the growth and early development of juvenile sporophytes. In vitro expression showed one distinct band existed at similar to 27 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results enriched the knowledge of AUREO properties in S. japonica and provided clues to explore the mechanisms underlying diverse physiological responses mediated by BL photoreceptors AUREO in the photosynthetic stramenopiles.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000331652200002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24124]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Deng, Yunyan,Yao, Jianting,Fu, Gang,et al. Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae)[J]. MARINE BIOTECHNOLOGY,2014,16(2):135-143.
APA	Deng, Yunyan,Yao, Jianting,Fu, Gang,Guo, Hui,&Duan, Delin.(2014).Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae).MARINE BIOTECHNOLOGY,16(2),135-143.
MLA	Deng, Yunyan,et al."Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae)".MARINE BIOTECHNOLOGY 16.2(2014):135-143.