Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways

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	Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways
	Duan, Hu 1,2; Jin, Songjun 1; Zhang, Yan 1; Li, Fuhua 1; Xiang, Jianhai1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2014-10-01
卷号	46 期号:2 页码:186-193
关键词	Granulocyte Endocytosis Beads E. coli WSSV Cherax quadricarinatus
通讯作者	Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	The hemocytes of the red claw crayfish Cherax quadricarinatus are classified by morphologic observation into the following types: hyalinocytes (H), semi-granulocytes (SG) and granulocytes (G). Density gradient centrifugation with Percoll was developed to separate these three subpopulations of hemocytes. Beads, Escherichia coli, and FITC labeling WSSV were used to investigate the characteristics of granulocytes by using scanning electron microscope, transmission electron microscope, and laser scan confocal microscope. Results showed that granulocytes could phagocytose beads and E. coli by endocytic pathways. WSSV could rely on caveolae-mediated endocytosis to mainly enter into granulocytes. These results could elucidate the mechanism of the innate immunity function of granulocytes, and it also showed the mechanism by which WSSV invaded granulocytes in the red claw crayfish. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Immunology; Zoology
收录类别	SCI
语种	英语
WOS记录号	WOS:000340216600009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24118]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Duan, Hu,Jin, Songjun,Zhang, Yan,et al. Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,46(2):186-193.
APA	Duan, Hu,Jin, Songjun,Zhang, Yan,Li, Fuhua,&Xiang, Jianhai.(2014).Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,46(2),186-193.
MLA	Duan, Hu,et al."Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 46.2(2014):186-193.