Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas

	Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas
	Song, Xiaorui1,2 ; Wang, Xiaotong 1; Li, Li 1; Zhang, Guofan 1
刊名	MOLECULAR BIOLOGY REPORTS
	2014-07-01
卷号	41 期号:7 页码:4273-4278
关键词	Nacrein-like proteins Shell biomineralization Crassostrea gigas Calcite fractions
通讯作者	Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China.
英文摘要	Nacrein-like proteins have carbonic anhydrase (CA)-like domains, but their coding regions are flanked by inserted repeat sequence, such as Gly-X-Asn. Reportedly, nacrein-like proteins show the highest similarity to human carbonic anhydrase 1(alpha-CA1), possess CA catalytic functions, and play a key role in shell biomineralization. In the present study, two novel nacrein-like proteins were firstly identified from the shell-forming mantle of the Pacific oyster Crassostrea gigas. With numerous analyses, it was identified and characterized that both the nacrein-like proteins F1 and F2 were secreted and most closely related to the nacrein-like protein of California mussel Mytilus californianus via phylogenetic analysis. RT-PCR analysis showed that the nacrein-like proteins F1 and F2 were expressed in multiple tissues and the expression levels remarkably rose after entering the spat stage, which were basically consistent with the increase of calcite fractions in the total shell volume. Surprisingly, the Gly-X-Asn repeat domain, which is distinctive in most nacrein-like proteins, was absent in the two newly identified nacrein-like proteins in C. gigas and replaced with a series of acidic amino acids (D/E). Regardless, nacrein-like proteins in mollusks seem to be vital to the deposition of calcium carbonate and likely perform diverse functions.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000338283000012
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24224]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Song, Xiaorui,Wang, Xiaotong,Li, Li,et al. Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas[J]. MOLECULAR BIOLOGY REPORTS,2014,41(7):4273-4278.
APA	Song, Xiaorui,Wang, Xiaotong,Li, Li,&Zhang, Guofan.(2014).Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas.MOLECULAR BIOLOGY REPORTS,41(7),4273-4278.
MLA	Song, Xiaorui,et al."Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas".MOLECULAR BIOLOGY REPORTS 41.7(2014):4273-4278.