Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus

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	Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus
	Ma Deyou 1,2; Yang Hongsheng1 ; Sun Lina 1,2; Xu Dongxue1,2
刊名	ACTA OCEANOLOGICA SINICA
	2014-08-01
卷号	33 期号:8 页码:55-61
关键词	454 cDNA sequencing albinism Apostichopus japonicus transcriptome
通讯作者	Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	The sea cucumber Apostichopus japonicus is an important economic species in China. Its dorsal body wall color is commonly tawny, whereas its ventral surface is fawn. Albino sea cucumbers are rarely observed. In order to profile gene expression and screen albinism-related genes, we compared the transcriptome of albino samples with a control by 454 cDNA sequencing. We found that 6 539 identified genes on the basis of sequence similarity to known genes were expressed in the albino A. japonicus. The gene ontology analysis indicated that the transcription of genes associated with the terms of biological regulation and pigmentation was non-abundant in the albino library compared to the control. Based on an analysis using the Kyoto Encyclopedia of Genes and Genomics (KEGG) database, we identified 14 important genes that were involved in major intercellular signaling pathways related to melanin synthesis, such as tyrosine metabolism, the mitogen-activated protein kinase (MAPK) pathway, and melanogenesis. The expressions of fibroblast growth factor receptor 4 (FGFR4), protein kinase C (PKC), protein kinase A (PKA), and Ras genes were significantly down-regulated in the albino transcriptome compared with the control, while the expressions of homogentisate 1, 2-dioxygenase gene (HGO), cAMP-responsive element binding protein (CREB), transcription factor AP-1(c-jun), and calmodulin (CaM) were significantly up-regulated (Fisher's exact test, p < 0.05). These differentially expressed genes could be candidate genes for revealing the mechanism of albinism and investigating regulation of melanin synthesis in A. japonicus.
学科主题	Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000339318800007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/23997]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ma Deyou,Yang Hongsheng,Sun Lina,et al. Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus[J]. ACTA OCEANOLOGICA SINICA,2014,33(8):55-61.
APA	Ma Deyou,Yang Hongsheng,Sun Lina,&Xu Dongxue.(2014).Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus.ACTA OCEANOLOGICA SINICA,33(8),55-61.
MLA	Ma Deyou,et al."Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus".ACTA OCEANOLOGICA SINICA 33.8(2014):55-61.