Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties

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	Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties
	Zhao, Caifeng 1; Zhang, Yewei 1; Wei, Xuetuan 2; Hu, Zhongbo 1; Zhu, Feiyan 1; Xu, Lin 2; Luo, Mingfang 3; Liu, Huizhou 2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2013-06-01
卷号	170 期号:3 页码:562-572
关键词	Bacillus licheniformis Fed-batch fermentation Flocculation gamma-Polyglutamic acid Ultra-high molecular weight
英文摘要	A novel strain of Bacillus licheniformis P-104 was isolated from Chinese soybean paste to produce a bioflocculant. The bioflocculant was confirmed as ultra-high molecular weight poly-gamma-glutamic acid (gamma-PGA) using Fourier transform infrared spectrum, high-performance liquid chromatography, and gel permeation chromatography with multi-angle laser light scattering. The production technology and flocculation properties of gamma-PGA were investigated. By fed-batch fermentation in a 7-L bioreactor, the maximum gamma-PGA yield reached 41.6 g L-1 with a productivity rate of 1.07 g L-1 h(-1). The flocculating activity of gamma-PGA for kaolin suspension was 33.5 +/- 1.6 1/OD under the optimized flocculation conditions (6 mM Ca2+, 1.5 mg L-1 gamma-PGA, and pH 6.0). The optimized dosage of gamma-PGA for flocculation was just about 30 % of that of reported gamma-PGA produced by other strains. Moreover, the flocculation activity of gamma-PGA produced by strain P-104 was much higher than commercial gamma-PGA with the molecular weight ranging 200-500 kDa and 1,500-2,500 kDa. This study provided a promising strain and an efficient method for production of ultra-high molecular weight gamma-PGA which could be used as a potential green bioflocculant.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	POLY(GAMMA-GLUTAMIC ACID) ; POLYGLUTAMIC ACID ; BIOPOLYMER FLOCCULANT ; KAOLIN SUSPENSION ; SUBTILIS ; BIOFLOCCULANT ; CONFORMATION ; AERATION ; CULTURES ; ISOLATE
收录类别	SCI
语种	英语
WOS记录号	WOS:000319134800008
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13538]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Technol Transfer Ctr, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Caifeng,Zhang, Yewei,Wei, Xuetuan,et al. Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,170(3):562-572.
APA	Zhao, Caifeng.,Zhang, Yewei.,Wei, Xuetuan.,Hu, Zhongbo.,Zhu, Feiyan.,...&Liu, Huizhou.(2013).Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,170(3),562-572.
MLA	Zhao, Caifeng,et al."Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170.3(2013):562-572.