Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization
Xin, Qian1; Jiang, Yanjun1,2; Gao, Jing1; Zhou, Liya1; Ma, Li1; He, Ying1; Jia, Fei1
刊名CHINESE JOURNAL OF CATALYSIS
2013-08-01
卷号34期号:8页码:1627-1633
关键词Composite microcapsule Layer-by-layer assembly Biomimetic mineralization Glucose oxidase
英文摘要Organic-inorganic composite microcapsules made of sodium poly(styrenesulfonate) (PSS), poly(dimethyl diallyl ammonium chloride) (PDADMAC), and biomimetic silica were successfully fabricated via a combination of layer-by-layer self-assembly and biomimetic mineralization. The morphologies and chemical compositions of the microcapsules (denoted by (PSS/PDADMAC)(2)-SiO2) were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The composite microcapsules were used as carrier for the immobilization of glucose oxidase (GOD). GOD encapsulated in (PSS/PDADMAC)(2)-SiO2 (denoted by GOD@(PSS/PDADMAC)(2)-SiO2) displayed improved thermal, pH, and operational stabilities. The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions. GOD@(PSS/PDADMAC)(2)-SiO2 showed a 2.21-fold increase in the Michaelis constant (K-m) compared with free GOD. The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems, and as biosensors. (C) 2013, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Applied ; Chemistry, Physical ; Engineering, Chemical
研究领域[WOS]Chemistry ; Engineering
关键词[WOS]DIRECT ELECTRON-TRANSFER ; CARBON NANOTUBES ; SILICA ; TITANIA ; BIOMINERALIZATION ; ELECTROCHEMISTRY ; MICROPARTICLES ; MINERALIZATION ; ENCAPSULATION ; PERFORMANCE
收录类别SCI
语种英语
WOS记录号WOS:000323239600020
公开日期2015-05-27
内容类型期刊论文
源URL[http://ir.ipe.ac.cn/handle/122111/13452]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Xin, Qian,Jiang, Yanjun,Gao, Jing,et al. Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization[J]. CHINESE JOURNAL OF CATALYSIS,2013,34(8):1627-1633.
APA Xin, Qian.,Jiang, Yanjun.,Gao, Jing.,Zhou, Liya.,Ma, Li.,...&Jia, Fei.(2013).Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization.CHINESE JOURNAL OF CATALYSIS,34(8),1627-1633.
MLA Xin, Qian,et al."Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization".CHINESE JOURNAL OF CATALYSIS 34.8(2013):1627-1633.
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