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	Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization
	Luan, Pan-Pan1,2; Jiang, Yan-Jun2; Zhang, Song-Ping1,3; Gao, Jing2; Su, Zhi-Guo1; Ma, Guang-Hui1; Zhang, Yu-Fei1
刊名	JOURNAL OF BIOSCIENCE AND BIOENGINEERING
	2014-11-01
卷号	118期号:5页码:575-582
关键词	Chitosan Biomimetic silicification Manganese peroxidase Silica nanoparticle Immobilization
ISSN号	1389-1723
其他题名	J. Biosci. Bioeng.
中文摘要	Our work here, for the first time, reported the use of chitosan-mediated biomimetic silica nanoparticles in enzyme immobilization. In order to make clear the relationship among silicification process, silica nanoparticle structure and immobilized enzyme activity, a mechanism of chitosan-mediated silicification using sodium silicate as the silica source was primarily evaluated. Chitosan was demonstrated effectively to promote the silicification not only in accelerating the aggregation rate of sodium silicate, but also in templating the formation of silica nanoparticles. Although the whole biomimetic silicification process contained polycondensation-aggregation precipitation three stages, the elemental unit in precipitated silica was confirmed to be nanoparticles with 100 nm diameter regardless of the chitosan and silicate concentration used. Furthermore, the effect of enzyme on silicification process was also investigated. The introducing of manganese peroxidase (MnP) to silica precursor solution had no obvious effect on the silicification rate and nanoparticle morphology. The residual activity and embedding rate of immobilized MnP were 64.2% and 36.4% respectively under the optimum conditions. In addition, compared to native MnP, the MnP embedded in chitosan/silica nanoparticles exhibited improved stability against organic solvent and ultrasonic wave. After ultrasonic treatment for 20 min, 77% of the initial activity was remained due to the protective effect of chitosan/silica nanoparticles, while native MnP lost almost all of its original activity. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
英文摘要	Our work here, for the first time, reported the use of chitosan-mediated biomimetic silica nanoparticles in enzyme immobilization. In order to make clear the relationship among silicification process, silica nanoparticle structure and immobilized enzyme activity, a mechanism of chitosan-mediated silicification using sodium silicate as the silica source was primarily evaluated. Chitosan was demonstrated effectively to promote the silicification not only in accelerating the aggregation rate of sodium silicate, but also in templating the formation of silica nanoparticles. Although the whole biomimetic silicification process contained polycondensation-aggregation precipitation three stages, the elemental unit in precipitated silica was confirmed to be nanoparticles with 100 nm diameter regardless of the chitosan and silicate concentration used. Furthermore, the effect of enzyme on silicification process was also investigated. The introducing of manganese peroxidase (MnP) to silica precursor solution had no obvious effect on the silicification rate and nanoparticle morphology. The residual activity and embedding rate of immobilized MnP were 64.2% and 36.4% respectively under the optimum conditions. In addition, compared to native MnP, the MnP embedded in chitosan/silica nanoparticles exhibited improved stability against organic solvent and ultrasonic wave. After ultrasonic treatment for 20 min, 77% of the initial activity was remained due to the protective effect of chitosan/silica nanoparticles, while native MnP lost almost all of its original activity. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]	COLLOIDAL SILICA ; DIATOM BIOSILICA ; ORGANIC MATRIX ; IN-VITRO ; SILICIFICATION ; PH ; DEGRADATION ; ANTHRACENE ; SEPARATION ; PEPTIDES
收录类别	SCI
原文出处	://WOS:000347141300015
语种	英语
WOS记录号	WOS:000347141300015
公开日期	2015-04-01
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/11803]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China 3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
推荐引用方式 GB/T 7714	Luan, Pan-Pan,Jiang, Yan-Jun,Zhang, Song-Ping,et al. Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2014,118(5):575-582.
APA	Luan, Pan-Pan.,Jiang, Yan-Jun.,Zhang, Song-Ping.,Gao, Jing.,Su, Zhi-Guo.,...&Zhang, Yu-Fei.(2014).Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,118(5),575-582.
MLA	Luan, Pan-Pan,et al."Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 118.5(2014):575-582.

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