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	Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro
	Liu, Yuying1,2; Chen, Xiaoming1,3; Wang, Lianyan1; Yang, Tingyuan1; Yuan, Qipeng2; Ma, Guanghui1
刊名	PARTICUOLOGY
	2014-12-01
卷号	17期号:DEC页码:74-80
关键词	Microparticle (MP) Macrophage Surface charge Antigen loading Phagocytosis Activation
ISSN号	1674-2001
其他题名	Particuology
中文摘要	The use of microparticles (MPs) as adjuvants has attracted increasing interest in vaccine delivery systems. Many physiochemical characteristics of MPs including hydrodynamic size, surface properties, and morphology can regulate the immune response. Surface charge is an important characteristic of MPs, but how it affects their adjuvanticity remains unknown. In this study, we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge, and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro. A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages, promoted the expression of MHC II and CD80, and increased the secretion level of TNF-alpha. Taken together, these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs. (C) 2014 Published by Elsevier B.V. on behalf of Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences.
英文摘要	The use of microparticles (MPs) as adjuvants has attracted increasing interest in vaccine delivery systems. Many physiochemical characteristics of MPs including hydrodynamic size, surface properties, and morphology can regulate the immune response. Surface charge is an important characteristic of MPs, but how it affects their adjuvanticity remains unknown. In this study, we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge, and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro. A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages, promoted the expression of MHC II and CD80, and increased the secretion level of TNF-alpha. Taken together, these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs. (C) 2014 Published by Elsevier B.V. on behalf of Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Chemical ; Materials Science, Multidisciplinary
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	COMPLEMENT ACTIVATION ; PARTICLE-SIZE ; MICROSPHERES ; ADJUVANTS ; RESPONSES
收录类别	SCI
原文出处	://WOS:000347656700012
语种	英语
WOS记录号	WOS:000347656700012
公开日期	2015-04-01
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/11801]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Univ Chem Technol, Coll Life Sci & Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuying,Chen, Xiaoming,Wang, Lianyan,et al. Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro[J]. PARTICUOLOGY,2014,17(DEC):74-80.
APA	Liu, Yuying,Chen, Xiaoming,Wang, Lianyan,Yang, Tingyuan,Yuan, Qipeng,&Ma, Guanghui.(2014).Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro.PARTICUOLOGY,17(DEC),74-80.
MLA	Liu, Yuying,et al."Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro".PARTICUOLOGY 17.DEC(2014):74-80.

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