KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans

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	KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans
	Chen, Xin; Ruan, Mei-Yu; Cai, Shi-Qing
刊名	JOURNAL OF NEUROSCIENCE
	2015
卷号	35 期号:5 页码:1880-1891
关键词	POTASSIUM CHANNELS K+ CHANNEL CARDIAC-ARRHYTHMIA PRIMARY CULTURE INACTIVATION NEURONS FAMILY MODULATION DEPENDENCE COMPLEXES
通讯作者	Cai, SQ (reprint author), 320 Yueyang Rd,Biol Res Bldg,Room 505A, Shanghai 200031, Peoples R China.
英文摘要	Voltage-gated Kv4 channels control the excitability of neurons and cardiac myocytes by conducting rapidly activating-inactivating currents. The function of Kv4 channels is profoundly modulated by K+ channel interacting protein (KChIP) soluble auxiliary subunits. However, the in vivo mechanism of the modulation is not fully understood. Here, we identified three C. elegans KChIP-like (ceKChIP) proteins, NCS-4, NCS-5, and NCS-7. All three ceKChIPs alter electrical characteristics of SHL-1, a C. elegans Kv4 channel ortholog, currents by slowing down inactivation kinetics and shifting voltage dependence of activation to more hyperpolarizing potentials. Native SHL-1 current is completely abolished in cultured myocytes of Triple KO worms in which all three ceKChIP genes are deleted. Reexpression of NCS-4 partially restored expression of functional SHL-1 channels, whereas NCS-4(efm), a NCS-4 mutant with impaired Ca2+-binding ability, only enhanced expression of SHL-1 proteins, but failed to transport them from the Golgi apparatus to the cell membrane in body wall muscles of Triple KO worms. Moreover, translational reporter revealed that NCS-4 assembles with SHL-1 K+ channels in male diagonal muscles. Deletion of either ncs-4 or shl-1 significantly impairs male turning, a behavior controlled by diagonal muscles during mating. The phenotype of the ncs-4 null mutant could be rescued by reexpression of NCS-4, but not NCS-4(efm), further emphasizing the importance of Ca2+ binding to ceKChIPs in regulating native SHL-1 channel function. Together, these data reveal an evolutionarily conserved mechanism underlying the regulation of Kv4 channels by KChIPs and unravel critical roles of ceKChIPs in regulating muscle cell excitability and animal behavior in C. elegans.
学科主题	Neurosciences
WOS标题词	Neurosciences & Neurology
语种	英语
WOS记录号	WOS:000349671100007
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2663]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Chen, Xin,Ruan, Mei-Yu,Cai, Shi-Qing. KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans[J]. JOURNAL OF NEUROSCIENCE,2015,35(5):1880-1891.
APA	Chen, Xin,Ruan, Mei-Yu,&Cai, Shi-Qing.(2015).KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans.JOURNAL OF NEUROSCIENCE,35(5),1880-1891.
MLA	Chen, Xin,et al."KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans".JOURNAL OF NEUROSCIENCE 35.5(2015):1880-1891.