Comparison of Vibrio and firefly luciferases as reporter gene systems for use in bacteria and plants

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 植物活体成像系统

	Comparison of Vibrio and firefly luciferases as reporter gene systems for use in bacteria and plants
	Mudge, S. R.
刊名	Australian Journal of Plant Physiology
	1996
卷号	23 期号:1 页码:75-83
ISSN号	0310-7841
中文摘要	Luciferase genes from Vibrio harveyi (luxAB) and firefly (luc) were introduced into E. coli, Agrobacterium, Arabidopsis and tobacco. Transformed bacteria and plants were quantitatively assayed for luciferase activity using a range of in vitro and in vivo assay conditions. Both lux and luc proved efficient reporter genes in bacteria, although it is important to be aware that the sensitive assays may detect expression due to readthrough from distant promoters. LUX activity was undetectable by liquid nitrogen-cooled CCD camera assays on intact tissues of plants which showed strong luxAB expression by in vitro assays. The decanal substrate for the lux assay was toxic to many plant tissues, and caused chemiluminescence in untransformed Arabidopsis leaves. These are serious limitations to application of the lux system for sensitive, non-toxic assays of reporter gene expression in plants. In contrast, LUC activity was readily detectable in intact tissues of all plants with luc expression detectable by luminometer assays on cell extracts. Image intensities of he-expressing leaves were commonly two to four orders of magnitude above controls under the CCD camera. Provided adequate penetration of the substrate luciferin is obtained, luc is suitable for applications requiring sensitive, non-toxic assays of reporter gene expression in plants.
语种	英语
公开日期	2015-04-03
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8508]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_植物活体成像系统
推荐引用方式 GB/T 7714	Mudge, S. R.. Comparison of Vibrio and firefly luciferases as reporter gene systems for use in bacteria and plants[J]. Australian Journal of Plant Physiology,1996,23(1):75-83.
APA	Mudge, S. R..(1996).Comparison of Vibrio and firefly luciferases as reporter gene systems for use in bacteria and plants.Australian Journal of Plant Physiology,23(1),75-83.
MLA	Mudge, S. R.."Comparison of Vibrio and firefly luciferases as reporter gene systems for use in bacteria and plants".Australian Journal of Plant Physiology 23.1(1996):75-83.