Three-dimensional visualization of rat brain microvasculature following permanent focal ischaemia by synchrotron radiation

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	Three-dimensional visualization of rat brain microvasculature following permanent focal ischaemia by synchrotron radiation
	Zhang, MQ ; Sun, DN ; Xie, YY ; Peng, GY ; Xia, J ; Long, HY ; Xiao, B
刊名	BRITISH JOURNAL OF RADIOLOGY
	2014
卷号	87 期号:1038
关键词	CEREBRAL-ARTERY OCCLUSION IN-VIVO ANGIOGENESIS ANGIOGRAPHY MICROANGIOGRAPHY REPERFUSION EXPRESSION MOUSE
ISSN号	0007-1285
通讯作者	xiaobo_xy@126.com
英文摘要	Objective: Identifying morphological changes that occur in microvessels under both normal and ischaemic conditions is crucial for understanding and treating stroke. However, conventional imaging techniques are not able to detect microvessels on a micron or sub-micron scale without angiography. In the present study, synchrotron radiation (SR)-based X-ray in-line phase contrast imaging (ILPCI) was used to acquire high-resolution and high-contrast images of rat brain tissues in both normal and ischaemic states. Methods: ILPCI was performed at the Shanghai Synchrotron Radiation Facility, Shanghai, China, without the use of contrast agents. CT slices were reformatted and then converted into three-dimensional (3D) reconstruction images to analyse subtle details of the cerebral microvascular network. Results: By using ILPCI, brain vessels up to 11.8 mu m in diameter were resolved. The number of cortical and penetrating arteries detected were found to undergo a remarkable decrease within the infarct area. 3 days after permanent ischaemia, vascular masses were also observed in the peripheral region of the infarcts. Conclusion: SR-based ILPCI-CT can serve as a powerful tool to accurately visualize brain microvasculature. The morphological parameters of blood vessels in both CT slices and 3D reconstructions were determined, and this approach has great potential for providing an effective diagnosis and evaluation for rehabilitation therapy for stroke. Advances in knowledge: In the absence of contrast agent, the 3D morphologies of the brain microvasculature in normal and stroke rats were obtained using SR-based ILPCI. SR imaging is a sensitive and promising method which can be used to explore primary brain function.
收录类别	SCI
语种	英语
WOS记录号	WOS:000339256600005
公开日期	2015-03-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/14512]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhang, MQ,Sun, DN,Xie, YY,et al. Three-dimensional visualization of rat brain microvasculature following permanent focal ischaemia by synchrotron radiation[J]. BRITISH JOURNAL OF RADIOLOGY,2014,87(1038).
APA	Zhang, MQ.,Sun, DN.,Xie, YY.,Peng, GY.,Xia, J.,...&Xiao, B.(2014).Three-dimensional visualization of rat brain microvasculature following permanent focal ischaemia by synchrotron radiation.BRITISH JOURNAL OF RADIOLOGY,87(1038).
MLA	Zhang, MQ,et al."Three-dimensional visualization of rat brain microvasculature following permanent focal ischaemia by synchrotron radiation".BRITISH JOURNAL OF RADIOLOGY 87.1038(2014).