Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area
	Han, Xue-Mei ; Liu, Yu-Rong ; Zheng, Yuan-Ming ; Zhang, Xiao-Xia ; He, Ji-Zheng
刊名	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
	2014
卷号	21 期号:16 页码:9754-9763
关键词	Polycyclic aromatic hydrocarbon Bioindicator qPCR PAH-degrading gene Persistent organic pollutants Soil pollution
ISSN号	0944-1344
中文摘要	Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is threatening human health and environmental safety. Investigating the relative prevalence of different PAH-degrading genes in PAH-polluted soils and searching for potential bioindicators reflecting the impact of PAH pollution on microbial communities are useful for microbial monitoring, risk evaluation, and potential bioremediation of soils polluted by PAHs. In this study, three functional genes, pdo1, nah, and C12O, which might be involved in the degradation of PAHs from a coke factory, were investigated by real-time quantitative PCR (qPCR) and clone library approaches. The results showed that the pdo1 and C12O genes were more abundant than the nah gene in the soils. There was a significantly positive relationship between the nah or pdo1 gene abundances and PAH content, while there was no correlation between C12O gene abundance and PAH content. Analyses of clone libraries showed that all the pdo1 sequences were grouped into Mycobacterium, while all the nah sequences were classified into three groups: Pseudomonas, Comamonas, and Polaromonas. These results indicated that the abundances of nah and pdo1 genes were positively influenced by levels of PAHs in soil and could be potential microbial indicators reflecting the impact of soil PAH pollution and that Mycobacteria were one of the most prevalent PAHs degraders in these PAH-polluted soils. Principal component analysis (PCA) and correlation analyses between microbial parameters and environmental factors revealed that total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) had positive effects on the abundances of all PAH-degrading genes. It suggests that increasing TC, TN, and DOC inputs could be a useful way to remediate PAH-polluted soils.
WOS记录号	WOS:000340613200031
公开日期	2015-03-23
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9221]
专题	生态环境研究中心_城市与区域生态国家重点实验室生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Han, Xue-Mei,Liu, Yu-Rong,Zheng, Yuan-Ming,et al. Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2014,21(16):9754-9763.
APA	Han, Xue-Mei,Liu, Yu-Rong,Zheng, Yuan-Ming,Zhang, Xiao-Xia,&He, Ji-Zheng.(2014).Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,21(16),9754-9763.
MLA	Han, Xue-Mei,et al."Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 21.16(2014):9754-9763.