Functional characterization of two microsomal fatty acid desaturases from Jatropha curcas L

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	Functional characterization of two microsomal fatty acid desaturases from Jatropha curcas L
	Wu, P. Z.
刊名	Journal of Plant Physiology
	2013
卷号	170 期号:15 页码:1360-1366
ISSN号	0176-1617
中文摘要	Linoleic acid (LA, C18:2) and alpha-linolenic acid (ALA, C18:3) are polyunsaturated fatty acids (PUFAs) and major storage compounds in plant seed oils. Microsomal omega-6 and omega-3 fatty acid (FA) desaturases catalyze the synthesis of seed oil LA and ALA, respectively. Jatropha curcas L seed oils contain large proportions of LA, but very little ALA. In this study, two microsomal desaturase genes, named JcFAD2 and JcFAD3, were isolated from J. curcas. Both deduced amino acid sequences possessed eight histidines shown to be essential for desaturases activity, and contained motif in the C-terminal for endoplasmic reticulum localization. Heterologous expression in Saccharomyces cerevisiae and Arabidopsis thaliana confirmed that the isolated JcFAD2 and JcFAD3 proteins could catalyze LA and ALA synthesis, respectively. The results indicate that JcFAD2 and JcFAD3 are functional in controlling PUFA contents of seed oils and could be exploited in the genetic engineering of J. curcas, and potentially other plants. (C) 2013 Elsevier GmbH. All rights reserved.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7937]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Wu, P. Z.. Functional characterization of two microsomal fatty acid desaturases from Jatropha curcas L[J]. Journal of Plant Physiology,2013,170(15):1360-1366.
APA	Wu, P. Z..(2013).Functional characterization of two microsomal fatty acid desaturases from Jatropha curcas L.Journal of Plant Physiology,170(15),1360-1366.
MLA	Wu, P. Z.."Functional characterization of two microsomal fatty acid desaturases from Jatropha curcas L".Journal of Plant Physiology 170.15(2013):1360-1366.