Complement C5a acts as molecular adjuvant in fish by enhancing antibody response to soluble antigen

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Complement C5a acts as molecular adjuvant in fish by enhancing antibody response to soluble antigen
	Wu, Nan 1; LaPatra, Scott E.2; Li, Jun 3,4; Sunyer, J. Oriol 3; Zhang, Yong-An 1,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014-10-01
卷号	40 期号:2 页码:616-623
关键词	C5a Complement Molecular adjuvant Rainbow trout IHNV
ISSN号	1050-4648
英文摘要	C5a, the most potent anaphylatoxin generated during complement activation, has important proinflammatory actions and has also been shown to enhance antigen-specific antibody response in mammals, thereby acting as a molecular adjuvant. In rainbow trout, C5a has been shown to have a chemoattractant ability and its receptor has also been found on potential APCs. In this study, we tested the possible role of trout C5a as a molecular adjuvant. We demonstrated the presence of native C5a in trout serum using the antibody generated by recombinant trout C5a, and then we generated recombinant infectious hematopoietic necrosis virus glycoprotein (G), and a G-C5a fusion protein to test the adjuvant activity of trout C5a. Recombinant G-C5a displayed a potent chemoattractant activity in contrast to G alone, indicating that the C5a portion of the fusion protein was functional. Thereafter, G-C5a, partially emulsified in a small quantity of IFA, was injected into one group of trout, while the other group of trout was inoculated with the same dose of recombinant G. At four to sixteen weeks post-injection, the serum IgM antibody levels of the fish injected with recombinant G-C5a were obviously higher than those injected with G protein alone. Thus, these results suggest, for the first time, that C5a acts as molecular adjuvant in teleost fish by enhancing antibody response to a soluble antigen. (C) 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	TROUT ONCORHYNCHUS-MYKISS ; HEMATOPOIETIC NECROSIS VIRUS ; RAINBOW-TROUT ; RESPIRATORY BURST ; TELEOST FISH ; LEUKOCYTE CHEMOTAXIS ; RECOMBINANT C5A ; ATLANTIC SALMON ; DNA VACCINATION ; IMMUNE-RESPONSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000343381100034
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20379]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Clear Spring Foods Inc, Div Res, Buhl, ID 83316 USA 3.Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA 4.Lake Super State Univ, Dept Biol Sci, Sault Ste Marie, MI 49783 USA
推荐引用方式 GB/T 7714	Wu, Nan,LaPatra, Scott E.,Li, Jun,et al. Complement C5a acts as molecular adjuvant in fish by enhancing antibody response to soluble antigen[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):616-623.
APA	Wu, Nan,LaPatra, Scott E.,Li, Jun,Sunyer, J. Oriol,&Zhang, Yong-An.(2014).Complement C5a acts as molecular adjuvant in fish by enhancing antibody response to soluble antigen.FISH & SHELLFISH IMMUNOLOGY,40(2),616-623.
MLA	Wu, Nan,et al."Complement C5a acts as molecular adjuvant in fish by enhancing antibody response to soluble antigen".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):616-623.