Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress

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	Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress
	[Wang, Yanhong ; Luo, Peng ; Zhang, Lvping ; Hu, Chaoqu ; Ren, Chunhua ; Xia, Jianjun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
刊名	MOLECULAR BIOLOGY REPORTS
	2013
卷号	40 期号:11 页码:6213-6221
关键词	Cloning Litopenaeus vannamei Quantitative real-time RT-PCR Rapid amplification of cDNA ends Sarco/endoplasmic reticulum Ca2+-ATPase
ISSN号	0301-4851
通讯作者	cqhu@scsio.ac.cn
中文摘要	Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) is an intracellular membrane bound enzyme that utilizes the free energy of ATP to transport Ca2+ against a concentration gradient. In the present study, a new SERCA gene (LvSERCA) from white shrimp (Litopenaeus vannamei) was cloned using suppression subtractive hybridization and rapid amplification of cDNA ends. The full-length cDNA of LvSERCA contained an open reading frame of 3,009 bp coding for 1,002 amino acids with a calculated molecular weight of approximately 109.8 kDa. The identity analysis of the amino acid sequence of LvSERCA showed that it is highly conserved with 10 transmembrane alpha-helices, one P-domain, one A-domain and one N-domain. The phylogenetic analysis revealed that LvSERCA is similar to other Arthropoda SERCA proteins. The mRNA levels of LvSERCA under salinity stress (3 and 40 g L-1) were analyzed by reverse transcription PCR and quantitative real-time PCR. The results showed that LvSERCA was expressed in all tissues detected. LvSERCA mRNA levels were significantly higher under hyper-salinity than hypo-salinity. These results highlight that Ga2+-ATPase plays an essential role in adjustment salinity stress, which may be useful for selective breeding of L. vannamei.
学科主题	Biochemistry & Molecular Biology
资助信息	This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270).
原文出处	SPRINGER
语种	英语
WOS记录号	WOS:000325948900018
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10971]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Wang, Yanhong,Luo, Peng,Zhang, Lvping,et al. Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress[J]. MOLECULAR BIOLOGY REPORTS,2013,40(11):6213-6221.
APA	[Wang, Yanhong,Luo, Peng,Zhang, Lvping,Hu, Chaoqu,Ren, Chunhua,&Xia, Jianjun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.(2013).Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress.MOLECULAR BIOLOGY REPORTS,40(11),6213-6221.
MLA	[Wang, Yanhong,et al."Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress".MOLECULAR BIOLOGY REPORTS 40.11(2013):6213-6221.