Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis
	Chan, Hsiu-Chien 1; Feng, Xinxin 2; Ko, Tzu-Ping 3; Huang, Chun-Hsiang 1; Hu, Yumei 1; Zheng, Yingying 1; Bogue, Shannon 2; Nakano, Chiaki 4; Hoshino, Tsutomu 4; Zhang, Lilan 1
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2014-02-19
卷号	136 期号:7 页码:2892-2896
英文摘要	We have obtained the structure of the bacterial diterpene synthase, tuberculosinol/iso-tuberculosinol synthase (Rv3378c) from Mycobacterium tuberculosis, a target for anti-infective therapies that block virulence factor formation. This phosphatase adopts the same fold as found in the Z- or cis-prenyltransferases. We also obtained structures containing the tuberculosinyl diphosphate substrate together with one bisphosphonate inhibitor-bound structure. These structures together with the results of site-directed mutagenesis suggest an unusual mechanism of action involving two Tyr residues. Given the similarity in local and global structure between Rv3378c and the M. tuberculosis cis-decaprenyl diphosphate synthase (DPPS; Rv2361c), the possibility exists for the development of inhibitors that target not only virulence but also cell wall biosynthesis, based in part on the structures reported here.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	DITERPENE CYCLASE ; EDAXADIENE ; VIRULENCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000331779800030
公开日期	2014-12-23
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/311007/545]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Univ Illinois, Dept Chem, Urbana, IL 61801 USA 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 4.Niigata Univ, Dept Appl Biol Chem, Niigata 9502181, Japan 5.Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA
推荐引用方式 GB/T 7714	Chan, Hsiu-Chien,Feng, Xinxin,Ko, Tzu-Ping,et al. Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2014,136(7):2892-2896.
APA	Chan, Hsiu-Chien.,Feng, Xinxin.,Ko, Tzu-Ping.,Huang, Chun-Hsiang.,Hu, Yumei.,...&Guo, Rey-Ting.(2014).Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,136(7),2892-2896.
MLA	Chan, Hsiu-Chien,et al."Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 136.7(2014):2892-2896.