QM/MM Study of the Reaction Mechanism of the Carboxyl Transferase Domain of Pyruvate Carboxylase from Staphylococcus aureus | |
Sheng, Xiang1; Liu, Yongjun1,2 | |
刊名 | biochemistry |
2014-07-15 | |
卷号 | 53期号:27页码:4455-4466 |
ISSN号 | 0006-2960 |
中文摘要 | pyruvate carboxylase (pc) catalyzes the carboxylation of pyruvate to produce oxaloacetate. its activity is directly related to insulin release and thus pc has recently attracted great interest as a potential target for diabetes treatment. in this article, the catalytic mechanism of the carboxyl transferase domain of pc from staphylococcus aureus was investigated by using a combined quantum-mechanical/molecular-mechanical approach. our calculation results indicate that the catalytic reaction starts from the decarboxylation of carboxybiotin to generate an enol-bti intermediate, followed by two consecutive proton-transfer processes (from t908 to enol-bti and from pyr to t908). during the catalytic reaction, the main-chain amide of t908 plays a key role in catching co2 and preventing its diffusion from the active center. a triad of residues, r571, q575, and k741, contributes both to substrate binding and enol-pyruvate stabilization. the oxyanion hole, consisting of the side-chain hydroxyl of s911 and the side-chain amino of q870, plays an important role in stabilizing the hydroxyl anion of bti. the coordination of the metal cation by pyruvate is a second sphere, rather than an inner sphere, interaction, and the metal cation stabilizes the species through the medium of residue k741. the decarboxylation of carboxybiotin corresponds to the highest free energy barrier of 21.7 kcal/mol. our results may provide useful information for both the regulation of enzyme activity and the development of related biocatalytic applications. |
英文摘要 | pyruvate carboxylase (pc) catalyzes the carboxylation of pyruvate to produce oxaloacetate. its activity is directly related to insulin release and thus pc has recently attracted great interest as a potential target for diabetes treatment. in this article, the catalytic mechanism of the carboxyl transferase domain of pc from staphylococcus aureus was investigated by using a combined quantum-mechanical/molecular-mechanical approach. our calculation results indicate that the catalytic reaction starts from the decarboxylation of carboxybiotin to generate an enol-bti intermediate, followed by two consecutive proton-transfer processes (from t908 to enol-bti and from pyr to t908). during the catalytic reaction, the main-chain amide of t908 plays a key role in catching co2 and preventing its diffusion from the active center. a triad of residues, r571, q575, and k741, contributes both to substrate binding and enol-pyruvate stabilization. the oxyanion hole, consisting of the side-chain hydroxyl of s911 and the side-chain amino of q870, plays an important role in stabilizing the hydroxyl anion of bti. the coordination of the metal cation by pyruvate is a second sphere, rather than an inner sphere, interaction, and the metal cation stabilizes the species through the medium of residue k741. the decarboxylation of carboxybiotin corresponds to the highest free energy barrier of 21.7 kcal/mol. our results may provide useful information for both the regulation of enzyme activity and the development of related biocatalytic applications. |
WOS标题词 | science & technology ; life sciences & biomedicine |
类目[WOS] | biochemistry & molecular biology |
研究领域[WOS] | biochemistry & molecular biology |
关键词[WOS] | molecular-dynamics simulation ; biotin-dependent enzymes ; decarboxylase na+ pump ; oxaloacetate decarboxylase ; catalytic mechanism ; enzymatic-reactions ; carboxyltransferase domain ; magnetic-resonance ; transition-state ; rhizobium-etli |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000339226500015 |
公开日期 | 2014-12-19 |
内容类型 | 期刊论文 |
源URL | [http://ir.nwipb.ac.cn/handle/363003/4209] |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
作者单位 | 1.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Sheng, Xiang,Liu, Yongjun. QM/MM Study of the Reaction Mechanism of the Carboxyl Transferase Domain of Pyruvate Carboxylase from Staphylococcus aureus[J]. biochemistry,2014,53(27):4455-4466. |
APA | Sheng, Xiang,&Liu, Yongjun.(2014).QM/MM Study of the Reaction Mechanism of the Carboxyl Transferase Domain of Pyruvate Carboxylase from Staphylococcus aureus.biochemistry,53(27),4455-4466. |
MLA | Sheng, Xiang,et al."QM/MM Study of the Reaction Mechanism of the Carboxyl Transferase Domain of Pyruvate Carboxylase from Staphylococcus aureus".biochemistry 53.27(2014):4455-4466. |
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