Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100

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	Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100
	Zhang, Yu1,2 ; Xu, Jing-Liang 1; Li, Dong 1; Yuan, Zhen-Hong1
刊名	biocatalysis and biotransformation
	2010-12-01
卷号	28 期号:5-6 页码:313-319
关键词	Immobilized cellulase Eudragit L-100 N-hydroxysuccinimide carbodiimide coupling smart biocatalysis
ISSN号	1024-2422
通讯作者	yuanzh@ms.giec.ac.cn
产权排序	[zhang, yu; xu, jing-liang; li, dong; yuan, zhen-hong] chinese acad sci, guangzhou inst energy & convers, key lab renewable energy & gas hydrate, guangzhou 510640, peoples r china; [zhang, yu] chinese acad sci, grad univ, beijing, peoples r china
中文摘要	to prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix eudragit l-100 by non-covalent and covalent methods. covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of n-hydroxysuccinimide. the temperature optimum of the cellulase was not improved apparently by immobilization but the ph optimum increased from 4.75 to 5.25. immobilized cellulase was more active than free cellulase above ph 5.0. immobilized cellulase was more stable than free cellulase during storage at 4 degrees c, room temperature and 50 degrees c. k(m) values of immobilized and free cellulase were 85.55 and 73.84 g l(-1), respectively. about 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
英文摘要	to prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix eudragit l-100 by non-covalent and covalent methods. covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of n-hydroxysuccinimide. the temperature optimum of the cellulase was not improved apparently by immobilization but the ph optimum increased from 4.75 to 5.25. immobilized cellulase was more active than free cellulase above ph 5.0. immobilized cellulase was more stable than free cellulase during storage at 4 degrees c, room temperature and 50 degrees c. k(m) values of immobilized and free cellulase were 85.55 and 73.84 g l(-1), respectively. about 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
学科主题	biochemistry & molecular biology ; biotechnology & applied microbiology
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology
研究领域[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology
关键词[WOS]	xylan-degrading enzymes ; bioethanol production ; enzymatic-hydrolysis ; polymer ; ethanol ; fermentation ; pretreatment ; carrageenan ; improvement ; chitosan
收录类别	SCI
资助信息	chinese academy of sciences [kscx-yw-11-a3, kscx2-yw-g-075-09, kgcx2-yw-335]; national high technology research and development program of china [2007aa05z406]
原文出处	https://dx.doi.org/10.3109/10242422.2010.516391
语种	英语
WOS记录号	WOS:000285554000003
公开日期	2014-12-24
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/8462]
专题	中国科学院广州能源研究所生物质能源生化转化实验室
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yu,Xu, Jing-Liang,Li, Dong,et al. Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100[J]. biocatalysis and biotransformation,2010,28(5-6):313-319.
APA	Zhang, Yu,Xu, Jing-Liang,Li, Dong,&Yuan, Zhen-Hong.(2010).Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100.biocatalysis and biotransformation,28(5-6),313-319.
MLA	Zhang, Yu,et al."Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100".biocatalysis and biotransformation 28.5-6(2010):313-319.