Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides

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	Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides
	Ji, H ; Wei, J ; Wei, S ; Yan, Y ; Huang, Y ; Huang, X ; Zhou, S ; Zhou, Y ; Qin, Q
刊名	JOURNAL OF FISH BIOLOGY
	2014
卷号	84 期号:2 页码:436-447
关键词	bacteria freezing-survival test grouper spleen cells haemagglutinating activity intracellular localization
ISSN号	0022-1112
通讯作者	qinqw@scsio.ac.cn
中文摘要	A C-type lectin-like protein (Ec-CTLP) was cloned from the grouper Epinephelus coioides. The full-length cDNA of Ec-CTLP was composed of 905 bp with a 522 bp open reading frame that encodes a 174-residue protein. The putative amino acid sequence of Ec-CTLP contains a signal peptide of 19 residues at the N-terminus and a CLECT domain from Cys43 to Arg169 and a conserved imperfect WND (Trp-Asn-Asp) motif. The homologous identity of deduced amino acid sequences is from 32 to 42% with other fishes. The expression of Ec-CTLP was differently upregulated in E. coioides spleen (germline stem) cells after being challenged at 16 and 4 degrees C. Intracellular localization revealed that Ec-CTLP was distributed only in the cytoplasm. Recombinant Ec-CTLP (rEc-CTLP) was expressed in Escherichia coli BL21 (DE3) and purified for mouse Mus musculus anti-Ec-CTLP serum preparation. The rEc-CTLP fusion protein does not possess haemagglutinating activity, but improves survival from frozen bacteria. The survival of bacteria (including gram-negative E. coli and gram-positive Staphylococcus aureus) was positively correlated with the concentration of the rEc-CTLP. These findings can provide clues to help understand the probable C-type lectin in marine fish innate immunity. (C) 2014 The Fisheries Society of the British Isles
学科主题	Fisheries ; Marine & Freshwater Biology
收录类别	SCI
资助信息	National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018]
原文出处	WILEY-BLACKWELL
WOS记录号	WOS:000336083200011
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10471]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Ji, H,Wei, J,Wei, S,et al. Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides[J]. JOURNAL OF FISH BIOLOGY,2014,84(2):436-447.
APA	Ji, H.,Wei, J.,Wei, S.,Yan, Y.,Huang, Y.,...&Qin, Q.(2014).Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides.JOURNAL OF FISH BIOLOGY,84(2),436-447.
MLA	Ji, H,et al."Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides".JOURNAL OF FISH BIOLOGY 84.2(2014):436-447.