Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities
	Li, PF ; Yan, Y ; Wei, SN ; Wei, JG ; Gao, R ; Huang, XH ; Huang, YH ; Jiang, GH ; Qin, QW
刊名	VIRUS RESEARCH
	2014
卷号	188 页码:146-154
关键词	DNA aptamers SELEX Grouper Antiviral agents SGIV
ISSN号	0168-1702
通讯作者	jgh982@bnu.edu.cn ; qinqw@scsio.ac.cn
中文摘要	The Singapore grouper iridovirus (SGIV), a member of the genus Ranavirus, is a major viral pathogen that has caused heavy economic losses to the grouper aquaculture industry in China and Southeast Asia. No efficient method of controlling SGIV outbreaks is currently available. Systematic evolution of ligands by exponential enrichment (SELEX) is now widely used for the in vitro selection of artificial ssDNA or RNA ligands, known as aptamers, which bind to targets through their stable three-dimensional structures. In our current study, we generated ssDNA aptamers against the SGIV, and evaluated their ability to block SGIV infection in cultured fish cells and cultured fish in vivo. The anti-SGIV DNA aptamers, LMB-761, LMB-764, LMB-748, LMB-439, LMB-755, and LMB-767, were selected from a pool of oligonucleotides randomly generated using a SELEX iterative method. The analysis of the secondary structure of the aptamers revealed that they all formed similar stem-loop structures. Electrophoretic mobility shift assays showed that the aptamers bound SGIV specifically, as evidenced by a lack cross-reactivity with the soft shell turtle iridovirus. The aptamers produced no cytotoxic effects in cultured grouper spleen cells (GS). Assessment of cytopathic effects (CPE) and viral titer assays showed that LMB-761, LMB-764, LMB-748, LMB-755, and LMB-767 significantly inhibited SGIV infection in GS cells. The in vivo experiments showed that LMB-761 and LMB-764 reduced SGIV-related mortality, and no negative effects were observed in orange-spotted grouper, Epinephelus coioides, indicating that these DNA aptamers may be suitable antiviral candidates for controlling SGIV infections in fish reared in marine aquaculture facilities. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Virology
收录类别	SCI
资助信息	National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [31330082]
原文出处	ELSEVIER SCIENCE BV
WOS记录号	WOS:000339128300019
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10404]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Li, PF,Yan, Y,Wei, SN,et al. Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities[J]. VIRUS RESEARCH,2014,188:146-154.
APA	Li, PF.,Yan, Y.,Wei, SN.,Wei, JG.,Gao, R.,...&Qin, QW.(2014).Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities.VIRUS RESEARCH,188,146-154.
MLA	Li, PF,et al."Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities".VIRUS RESEARCH 188(2014):146-154.