Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis
	Huang, YH ; Huang, XH ; Ouyang, ZL ; Wei, SN ; Guo, CY ; Qin, QW
刊名	AQUACULTURE
	2014
卷号	432 页码:265-272
关键词	Epinephelus lanceolatus ELGSN Snout Iridovirus Nodavirus Macropinocytosis
ISSN号	0044-8486
通讯作者	qinqw@scsio.ac.cn
中文摘要	We developed and characterized a new marine fish cell line (ELGSN) from the snout of giant grouper, Epinephelus lanceolatus (Bloch). Two different phenotypes of cells, ELGSN(e), which consisted predominantly of epithelial-like cells, and ELGSN(f), which consisted predominantly of fibroblast-like cells, were isolated and cultured. Both ELGSN, and ELGSN(f) cells were subcultured for >80 passages and multiplied well in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28 degrees C. Karyotyping analysis indicated that the modal chromosome numbers of ELGSN(e) and ELGSN(f) cells were 48 and 62, respectively. After transfection with EGFP-C1, bright green fluorescence was observed in these two cells and the transfection efficiency was 35% and 28%, respectively. Moreover, the two types of ELGSN cells both showed susceptibility to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), but not to soft-shelled turtle iridovirus (STIV) and spring viremia of carp virus (SVCV), indicated by the occurrence of severe cytopathic effect and increased viral titers. In SGIV-infected cells, paracrystalline arrays, different stages of virus particles and amorphous structures were observed under electron microscopy. In RGNNV-infected cells, numerous virus particles and circular virus-packed macropinosome-like structures were observed. All these data suggested that ELGSN cells could be used for effective virus propagation in vitro. In addition, we provided biochemical evidences that macropinocytosis was involved in RGNNV infection in ELGSN cells. Taken together, the new established ELGSN cell lines provided a useful tool for transgenic and genetic manipulation, as well as virus propagation and pathogenesis. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Fisheries ; Marine & Freshwater Biology
收录类别	SCI
资助信息	National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014]
原文出处	ELSEVIER SCIENCE BV
WOS记录号	WOS:000342247200034
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10399]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Huang, YH,Huang, XH,Ouyang, ZL,et al. Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis[J]. AQUACULTURE,2014,432:265-272.
APA	Huang, YH,Huang, XH,Ouyang, ZL,Wei, SN,Guo, CY,&Qin, QW.(2014).Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis.AQUACULTURE,432,265-272.
MLA	Huang, YH,et al."Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis".AQUACULTURE 432(2014):265-272.