Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology

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	Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology
	Li, LL ; Zhou, S ; Ji, HS ; Gao, R ; Qin, QW
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2014
卷号	32 期号:5 页码:1036-1045
关键词	1 Klebsiella pneumoniae HSL4 fermentation response surface methodology (RSM) optimization 3-propanediol (1 3-PDO)
ISSN号	0254-4059
通讯作者	zhous@scsio.ac.cn ; qinqw@scsio.ac.cn
中文摘要	The industrially important organic compound 1,3-propanediol (1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K-2 HPO4, 7.6 g/L (NH4)(2) SO4, 3.0 g/L KH2 PO4, pH 7.1, cultivation at 35A degrees C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L center dot h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.
学科主题	Marine & Freshwater Biology ; Oceanography
收录类别	SCI
资助信息	Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]
原文出处	SCIENCE PRESS
WOS记录号	WOS:000340879900009
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10390]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Li, LL,Zhou, S,Ji, HS,et al. Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(5):1036-1045.
APA	Li, LL,Zhou, S,Ji, HS,Gao, R,&Qin, QW.(2014).Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(5),1036-1045.
MLA	Li, LL,et al."Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.5(2014):1036-1045.