Antifungal effect and mechanism of garlic oil on Penicillium funiculosum

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	Antifungal effect and mechanism of garlic oil on Penicillium funiculosum
	Li, WR ; Shi, QS ; Liang, Q ; Huang, XM ; Chen, YB
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2014
卷号	98 期号:19 页码:8337-8346
关键词	Garlic oil Antifungal effects Antifungal mechanism Penicillium funiculosum
ISSN号	0175-7598
通讯作者	jigan@gdim.cn
中文摘要	Garlic oil is a kind of fungicide, but little is known about its antifungal effects and mechanism. In this study, the chemical constituents, antifungal activity, and effects of garlic oil were studied with Penicillium funiculosum as a model strain. Results showed that the minimum fungicidal concentrations (MFCs, v/v) were 0.125 and 0.0313 % in agar medium and broth medium, respectively, suggesting that the garlic oil had a strong antifungal activity. The main ingredients of garlic oil were identified as sulfides, mainly including disulfides (36 %), trisulfides (32 %) and monosulfides (29 %) by gas chromatograph-mass spectrometer (GC/MS), which were estimated as the dominant antifungal factors. The observation results by transmission electron microscope (TEM) and scanning electron microscope (SEM) indicated that garlic oil could firstly penetrate into hyphae cells and even their organelles, and then destroy the cellular structure, finally leading to the leakage of both cytoplasm and macromolecules. Further proteomic analysis displayed garlic oil was able to induce a stimulated or weakened expression of some key proteins for physiological metabolism. Therefore, our study proved that garlic oil can work multiple sites of the hyphae of P. funiculosum to cause their death. The high antifungal effects of garlic oil makes it a broad application prospect in antifungal industries.
学科主题	Biotechnology & Applied Microbiology
收录类别	SCI
资助信息	Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115]
原文出处	SPRINGER
WOS记录号	WOS:000342072500024
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10383]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Li, WR,Shi, QS,Liang, Q,et al. Antifungal effect and mechanism of garlic oil on Penicillium funiculosum[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(19):8337-8346.
APA	Li, WR,Shi, QS,Liang, Q,Huang, XM,&Chen, YB.(2014).Antifungal effect and mechanism of garlic oil on Penicillium funiculosum.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(19),8337-8346.
MLA	Li, WR,et al."Antifungal effect and mechanism of garlic oil on Penicillium funiculosum".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.19(2014):8337-8346.