A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers
	Gu, Dongyu; Yang, Yi; Bakri, Mahinur; Chen, Qibin; Xin, Xuelei; Aisa, Haji Akber
刊名	PHYTOCHEMICAL ANALYSIS
	2013
卷号	24 期号:6 页码:661-670
关键词	LC/QTOF-MS/MS flavonoids protein tyrosine phosphatase 1B inhibition tannins Rosa rugosa Thunb flowers
ISSN号	0958-0344
通讯作者	Aisa, HA
中文摘要	IntroductionRosa rugosa flowers used as herbal medicine possess many activities. A fraction extracted by ethyl acetate exhibited strong inhibitive activity against protein tyrosine phosphatase 1B (PTP1B) in vitro. ObjectiveEstablish an efficient method of LC coupled to quadrupole time-of-flight (QTOF) with tandem MS/MS to investigate the compositions in the active fraction. MethodsChemical compositions were separated and investigated by LC/QTOF-MS/MS in negative electrospray ionisation (ESI) mode at different collision energy (CE) values. The maximal structural information was obtained for the identification of components. ResultsA total of 75 compounds including tannins, their related compounds and flavonoids were identified or partially characterised according to accurate mass and the characteristic fragments at low and high CE. Meanwhile, the fragmentation pathways of gallotannins and ellagitannins (hexahydroxydiphenoyl group and lactonised valoneoyl group) were studied and proposed and were used to trace tannins in crude extracts. ConclusionThe results suggest that this fraction is a source of PTP1B inhibitory activity with a potential for treating diabetes. Copyright (c) 2013 John Wiley & Sons, Ltd. As a traditional herbal medicine, Rosa rugosa flowers possess many activities. In this study, a LC-MS method was established for investigation of chemical compositions in a fraction with the PTP 1B inhibitory activity from Rosa rugosa. And 75 chemical compositions in this fraction were identified or tentatively characterized. The fragmentation pathways of gallotannins and ellagitannins were proposed. Knowing the phytochemical profiles of PTP 1B inhibitory activity fraction of this plant gives insights to its potential application to promote health.
收录类别	SCI
资助信息	China National Science Fund for Distinguished Young Scholars CAS/SAFEA International Partnership Program for Creative Research Teams and Major State Basic Research Development Program
公开日期	2014-11-11
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/3858]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室新疆理化技术研究所_资源化学研究室
作者单位	Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
推荐引用方式 GB/T 7714	Gu, Dongyu,Yang, Yi,Bakri, Mahinur,et al. A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):661-670.
APA	Gu, Dongyu,Yang, Yi,Bakri, Mahinur,Chen, Qibin,Xin, Xuelei,&Aisa, Haji Akber.(2013).A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers.PHYTOCHEMICAL ANALYSIS,24(6),661-670.
MLA	Gu, Dongyu,et al."A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers".PHYTOCHEMICAL ANALYSIS 24.6(2013):661-670.