An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots

	An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots
	Sui, Tao 1; Ge, Yingbin 2; Liu, Wujun 3; Zhao, Zongbao K.3; Zhang, Ning 1; Cao, Xiaojian 1
刊名	plos one
	2013-07-23
卷号	8 期号:7 页码:69049
通讯作者	赵宗保 ; zhang n ; cao xj
产权排序	待补充
合作状况	英
英文摘要	differences in the levels of acetylcholinesterase (ache) in ventral and dorsal spinal roots can be used to differentiate the spinal nerves. although many methods are available to assay ache, a rapid and sensitive method has not been previously developed. here, we describe an antibody-based quartz crystal microbalance (qcm) assay and its application for the quantification of ache in the solutions of ventral and dorsal spinal roots. the frequency variation of the qcm device corresponds to the level of ache over a wide dynamic range (0.5-10 mu g/ml), which is comparable to the response range of the elisa method. the frequency shift caused by the ventral roots is 3-fold greater than that caused by the dorsal roots. the antibody-based qcm sensor was stable across many successive replicate samples, and the method required less than 10 min, including the ache extraction and analysis steps. this method is a rapid and convenient means for the quantification of ache in biological samples and may be applicable for distinguishing the ventral and dorsal roots during surgical operations.
学科主题	物理化学
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	peripheral-nerve ; histochemical identification ; immobilization methods ; sensory fascicles ; motor ; dissipation ; neurons ; immunohistochemistry ; nanoparticles ; immunosensor
收录类别	SCI
资助信息	3，3
原文出处	69049
语种	英语
WOS记录号	WOS:000325211000071
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119668]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nanjing Med Univ, Affiliated Hosp 1, Dept Orthoped, Nanjing, Jiangsu, Peoples R China 2.Nanjing Med Univ, Dept Physiol, Nanjing, Jiangsu, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Sui, Tao,Ge, Yingbin,Liu, Wujun,et al. An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots[J]. plos one,2013,8(7):69049.
APA	Sui, Tao,Ge, Yingbin,Liu, Wujun,Zhao, Zongbao K.,Zhang, Ning,&Cao, Xiaojian.(2013).An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots.plos one,8(7),69049.
MLA	Sui, Tao,et al."An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots".plos one 8.7(2013):69049.