Retention mechanism and enrichment of glycopeptides on titanium dioxide

	Retention mechanism and enrichment of glycopeptides on titanium dioxide
	Sheng, Qianying 1; Li, Xiuling 2; Yin, Wei 3; Yu, Long 2; Ke, Yanxiong 1; Liang, Xinmiao 1,2
刊名	analytical methods
	2013
卷号	5 期号:24 页码:7072-7080
通讯作者	李秀玲 ; 梁鑫淼
产权排序	待补充
合作状况	英
英文摘要	a uniform method for the facile and selective enrichment of glycopeptides with titanium dioxide (tio2) is presented. retention mechanisms between glycopeptides and tio2 were explored by checking the influence of acetonitrile content, nh3 center dot h2o concentration and formic acid concentration on the retention of glycopeptides. both hydrophilic interaction and ligand-exchange retention mechanisms between glycopeptides and tio2 were found to be involved. based on this, a uniform method was established for selective enrichment of glycopeptides with tio2. the developed method exhibited high selectivity for n-linked glycopeptides with short to long peptide sequences. this efficient and uniform method will be a valuable tool for post-translational modification in proteomics.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine ; technology
类目[WOS]	chemistry, analytical ; food science & technology ; spectroscopy
研究领域[WOS]	chemistry ; food science & technology ; spectroscopy
关键词[WOS]	hydrophilic interaction chromatography ; glycosylation ; matrix ; peptides ; hplc
收录类别	SCI
资助信息	2，2
原文出处	7080
语种	英语
WOS记录号	WOS:000327506600028
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119098]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Dalian Maritime Univ, Dalian 116026, Peoples R China
推荐引用方式 GB/T 7714	Sheng, Qianying,Li, Xiuling,Yin, Wei,et al. Retention mechanism and enrichment of glycopeptides on titanium dioxide[J]. analytical methods,2013,5(24):7072-7080.
APA	Sheng, Qianying,Li, Xiuling,Yin, Wei,Yu, Long,Ke, Yanxiong,&Liang, Xinmiao.(2013).Retention mechanism and enrichment of glycopeptides on titanium dioxide.analytical methods,5(24),7072-7080.
MLA	Sheng, Qianying,et al."Retention mechanism and enrichment of glycopeptides on titanium dioxide".analytical methods 5.24(2013):7072-7080.