A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation
	Feng, Lifang 1,2; Fu, Chengjie 1; Yuan, Dongxia 1; Miao, Wei 1
刊名	AQUATIC TOXICOLOGY
	2014-04-01
卷号	149 页码:126-132
关键词	Tetrahymena thermophila p450 gene DDT Degradation
ISSN号	0166-445X
通讯作者	Feng, LF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
中文摘要	Analysis of metabolic mechanisms of dichlorodiphenyltrichloroethane (DDT) accumulation and degradation in microorganisms, which could be used to reduce its hazard to higher organisms at the higher in the food chain, have not been investigated. Robust resistance to DDT (grows well in 256 mg/L DDT) and a surprising ability to degrade DDT (more than 70% DDT within 4 h) were found in the ciliated protozoan Tetrahymena thermophila. A P450 gene (CYP5013C2) was found to respond specifically to DDT treatment. In the presence of 256 mg/L DDT, cells with overexpressing CYP5013C2 (p450-OE) grew faster and degraded DDT more efficiently than wild-type (WT) cells, while cells with CYP5013C2 partially knocked down (p450-KD) grew slower and exhibited reduced ability to degrade DDT compared to \NT cells. Both dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DOD) were detected in cells after exposure to DDT, and the concentration of ODD in the p450-OE strain gradually decreased from 0.5 to 4h. Thus, we argue that this P450 gene (CYP5013C2), by efficiently degrading DDT to DDD, is associated with robust resistance to DDT in Tetrahymena, and that a strain overexpressing this gene has the potential to serve as bioreactor that degrades environmental DDT. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要	Analysis of metabolic mechanisms of dichlorodiphenyltrichloroethane (DDT) accumulation and degradation in microorganisms, which could be used to reduce its hazard to higher organisms at the higher in the food chain, have not been investigated. Robust resistance to DDT (grows well in 256 mg/L DDT) and a surprising ability to degrade DDT (more than 70% DDT within 4 h) were found in the ciliated protozoan Tetrahymena thermophila. A P450 gene (CYP5013C2) was found to respond specifically to DDT treatment. In the presence of 256 mg/L DDT, cells with overexpressing CYP5013C2 (p450-OE) grew faster and degraded DDT more efficiently than wild-type (WT) cells, while cells with CYP5013C2 partially knocked down (p450-KD) grew slower and exhibited reduced ability to degrade DDT compared to \NT cells. Both dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DOD) were detected in cells after exposure to DDT, and the concentration of ODD in the p450-OE strain gradually decreased from 0.5 to 4h. Thus, we argue that this P450 gene (CYP5013C2), by efficiently degrading DDT to DDD, is associated with robust resistance to DDT in Tetrahymena, and that a strain overexpressing this gene has the potential to serve as bioreactor that degrades environmental DDT. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology ; Toxicology
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
关键词[WOS]	SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; INSECTICIDE RESISTANCE ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; EXPRESSED GENES ; TRIBUTYLTIN TBT ; METABOLISM ; CYTOCHROME-P450 ; TRANSFORMATION ; DROSOPHILA ; TOXICITY
收录类别	SCI
资助信息	National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082]
语种	英语
WOS记录号	WOS:000334989300014
公开日期	2014-08-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20088]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Feng, Lifang,Fu, Chengjie,Yuan, Dongxia,et al. A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation[J]. AQUATIC TOXICOLOGY,2014,149:126-132.
APA	Feng, Lifang,Fu, Chengjie,Yuan, Dongxia,&Miao, Wei.(2014).A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation.AQUATIC TOXICOLOGY,149,126-132.
MLA	Feng, Lifang,et al."A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation".AQUATIC TOXICOLOGY 149(2014):126-132.