Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects
	Cheng, Xiaoxia 1,2; Chen, Xiaowen 1; Jin, Xia 1; He, Jiangyan 1; Yin, Zhan 1,3
刊名	TOXICOLOGY AND APPLIED PHARMACOLOGY
	2014-07-01
卷号	278 期号:1 页码:78-84
关键词	gsu alpha promoter Pituitary hormone Transgenic zebrafish gsu alpha expression Endocrine disrupting
ISSN号	0041-008X
通讯作者	Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
中文摘要	The glycoprotein subunit alpha(gsu alpha) gene encodes the shared a subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone beta (Fsh beta), luteinizing hormone beta (Lh beta) and thyroid stimulating hormone beta (Tsh beta). In our current study, we identified and characterized the promoter region of zebrafish gsu alpha and generated a stable gsu alpha:EGFP transgenic line, which recapitulated the endogenous gsu alpha expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsu alpha promoters. Our results also demonstrated that the expression patterns of the gsu alpha:EGFP transgene were all identical to those expression patterns of the endogenous gsu alpha expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KCIO4, dexamethasone (Dex),beta-estradiol and progesterone. Thus, this gsu alpha:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsu alpha-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well. (C) 2014 Elsevier Inc. All rights reserved.
英文摘要	The glycoprotein subunit alpha(gsu alpha) gene encodes the shared a subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone beta (Fsh beta), luteinizing hormone beta (Lh beta) and thyroid stimulating hormone beta (Tsh beta). In our current study, we identified and characterized the promoter region of zebrafish gsu alpha and generated a stable gsu alpha:EGFP transgenic line, which recapitulated the endogenous gsu alpha expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsu alpha promoters. Our results also demonstrated that the expression patterns of the gsu alpha:EGFP transgene were all identical to those expression patterns of the endogenous gsu alpha expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KCIO4, dexamethasone (Dex),beta-estradiol and progesterone. Thus, this gsu alpha:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsu alpha-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well. (C) 2014 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]	Pharmacology & Pharmacy ; Toxicology
关键词[WOS]	CRE-MEDIATED RECOMBINATION ; SUBUNIT GENE ; PITUITARY-GLAND ; EFFICIENT TRANSGENESIS ; HORMONE ; TRANSCRIPTION ; EXPRESSION ; RECEPTOR ; ADENOHYPOPHYSIS ; GONADOTROPES
收录类别	SCI
资助信息	National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701]
语种	英语
WOS记录号	WOS:000336887100011
公开日期	2014-08-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20082]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.State Key Lab Freshwater Ecol & Biotechnol, Ningbo Lab, Ningbo, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Xiaoxia,Chen, Xiaowen,Jin, Xia,et al. Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2014,278(1):78-84.
APA	Cheng, Xiaoxia,Chen, Xiaowen,Jin, Xia,He, Jiangyan,&Yin, Zhan.(2014).Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects.TOXICOLOGY AND APPLIED PHARMACOLOGY,278(1),78-84.
MLA	Cheng, Xiaoxia,et al."Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects".TOXICOLOGY AND APPLIED PHARMACOLOGY 278.1(2014):78-84.