Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches

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	Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches
	Zhang, Xiang 1,2; Feng, Weisong 1; Iran, Qingyun 1; Yu, Yuhe 1
刊名	JOURNAL OF PURE AND APPLIED MICROBIOLOGY
	2013-12-01
卷号	7 期号:4 页码:2731-2738
关键词	Free-living particle-attached DGGE pattern 16S rDNA bacterial groups
ISSN号	0973-7510
通讯作者	Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
中文摘要	To find out different sampling approaches how to affect DNA fingerprinting, environmental genomic DNA was prepared with three sampling approaches. The DNA profiles of 5 bacterial subgroups (alpha-,beta-,gamma-Proteobacteria, Bacteroidetes and Cyanobacteria) and total bacteria were investigated by using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The DGGE patterns showed alpha-Proteobacteria had more free-living OTUs, while gamma-Proteobacteria and Bacteroidetes had more particle-attached OTUs. Cyanobacteria and beta-Proteobacteria had approximately equal OTU number in the two fractions. Principle component analysis based on DNA banding patterns showed there were obvious differences among free-living, particle-attached fraction and whole bacterial community composition of 3 bacterial subgroups (alpha-, gamma-Proteobacteria and Bacteroidetes) and total bacteria, while the patterns of beta-Proteobacteria and Cyanobacteria were similar, suggesting the dynamics of bacterial subgroups can be better understand by combining different collection approaches.
英文摘要	To find out different sampling approaches how to affect DNA fingerprinting, environmental genomic DNA was prepared with three sampling approaches. The DNA profiles of 5 bacterial subgroups (alpha-,beta-,gamma-Proteobacteria, Bacteroidetes and Cyanobacteria) and total bacteria were investigated by using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The DGGE patterns showed alpha-Proteobacteria had more free-living OTUs, while gamma-Proteobacteria and Bacteroidetes had more particle-attached OTUs. Cyanobacteria and beta-Proteobacteria had approximately equal OTU number in the two fractions. Principle component analysis based on DNA banding patterns showed there were obvious differences among free-living, particle-attached fraction and whole bacterial community composition of 3 bacterial subgroups (alpha-, gamma-Proteobacteria and Bacteroidetes) and total bacteria, while the patterns of beta-Proteobacteria and Cyanobacteria were similar, suggesting the dynamics of bacterial subgroups can be better understand by combining different collection approaches.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	GRADIENT GEL-ELECTROPHORESIS ; LIVING BACTERIAL COMMUNITIES ; NW MEDITERRANEAN SEA ; 16S RIBOSOMAL-RNA ; ASSEMBLAGES ; DIVERSITY ; LAKE ; VARIABILITY ; DYNAMICS ; RIVER
收录类别	SCI
资助信息	National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05]
语种	英语
WOS记录号	WOS:000331428900030
公开日期	2014-08-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19967]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiang,Feng, Weisong,Iran, Qingyun,et al. Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches[J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY,2013,7(4):2731-2738.
APA	Zhang, Xiang,Feng, Weisong,Iran, Qingyun,&Yu, Yuhe.(2013).Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches.JOURNAL OF PURE AND APPLIED MICROBIOLOGY,7(4),2731-2738.
MLA	Zhang, Xiang,et al."Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches".JOURNAL OF PURE AND APPLIED MICROBIOLOGY 7.4(2013):2731-2738.