ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71

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	ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71
	Yu, Liqin 1,2; Liu, Chunsheng 1; Chen, Qi 1; Zhou, Bingsheng 1
刊名	ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
	2014-06-01
卷号	33 期号:6 页码:1354-1362
关键词	Polybrominated diphenyl ethers (PBDEs) Reproductive toxicity Endocrine disruption Sex hormone Zebrafish
ISSN号	0730-7268
通讯作者	Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
中文摘要	The objective of the present study was to investigate the impact of polybrominated diphenyl ethers (PBDEs) on fish reproduction over 2 generations. Zebrafish (Danio rerio) embryos (F0) were exposed to low concentrations (3 mu g/L, 10 mu g/L, and 30 mu g/L) of the PBDE mixture DE-71 until they were sexually mature, and steroid hormone production, expression of genes involved in steroidogenesis, gonadal development, and gamete characteristics were examined. Exposure of female zebrafish to DE-71 resulted in lower estradiol production and downregulation of cytochrome P450 aromatase mRNA. In males, exposure to DE-71 resulted in greater testosterone production and greater cytochrome P450 c17 -hydroxylase,17,20-lase mRNA expression. Moreover, hepatic vitellogenin mRNA and estrogenic receptor gene transcription were downregulated in females and males. Expression of the follicle-stimulating hormone gene in the pituitary was upregulated, and the expression of luteinizing hormone was downregulated in both sexes. Histological examination showed inhibition of oocyte maturation in females and retarded spermiation in males. The average number of eggs (F1) produced was also reduced. Additionally, exposure of F0 embryos to DE-71 did not result in developmental toxicity, whereas delayed hatching, reduced survival, and decreased growth were observed in the F1 embryos derived from parent fish exposed to DE-71. Therefore, long-term exposure to low concentrations of PBDEs in zebrafish could cause reproductive impairment, suggesting that PBDEs might have significant adverse effects on fish population in the highly PBDEs-contaminated aquatic environment. Environ Toxicol Chem 2014;33:1354-1362. (c) 2014 SETAC
英文摘要	The objective of the present study was to investigate the impact of polybrominated diphenyl ethers (PBDEs) on fish reproduction over 2 generations. Zebrafish (Danio rerio) embryos (F0) were exposed to low concentrations (3 mu g/L, 10 mu g/L, and 30 mu g/L) of the PBDE mixture DE-71 until they were sexually mature, and steroid hormone production, expression of genes involved in steroidogenesis, gonadal development, and gamete characteristics were examined. Exposure of female zebrafish to DE-71 resulted in lower estradiol production and downregulation of cytochrome P450 aromatase mRNA. In males, exposure to DE-71 resulted in greater testosterone production and greater cytochrome P450 c17 -hydroxylase,17,20-lase mRNA expression. Moreover, hepatic vitellogenin mRNA and estrogenic receptor gene transcription were downregulated in females and males. Expression of the follicle-stimulating hormone gene in the pituitary was upregulated, and the expression of luteinizing hormone was downregulated in both sexes. Histological examination showed inhibition of oocyte maturation in females and retarded spermiation in males. The average number of eggs (F1) produced was also reduced. Additionally, exposure of F0 embryos to DE-71 did not result in developmental toxicity, whereas delayed hatching, reduced survival, and decreased growth were observed in the F1 embryos derived from parent fish exposed to DE-71. Therefore, long-term exposure to low concentrations of PBDEs in zebrafish could cause reproductive impairment, suggesting that PBDEs might have significant adverse effects on fish population in the highly PBDEs-contaminated aquatic environment. Environ Toxicol Chem 2014;33:1354-1362. (c) 2014 SETAC
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	POLYBROMINATED DIPHENYL ETHERS ; H295R CELL-LINE ; MINNOW PIMEPHALES-PROMELAS ; KESTRELS FALCO-SPARVERIUS ; FATHEAD MINNOW ; DANIO-RERIO ; AMERICAN KESTRELS ; GENE-EXPRESSION ; IN-VIVO ; DEVELOPMENTAL NEUROTOXICITY
收录类别	SCI
资助信息	National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
语种	英语
WOS记录号	WOS:000335936900005
公开日期	2014-08-08
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20062]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yu, Liqin,Liu, Chunsheng,Chen, Qi,et al. ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2014,33(6):1354-1362.
APA	Yu, Liqin,Liu, Chunsheng,Chen, Qi,&Zhou, Bingsheng.(2014).ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,33(6),1354-1362.
MLA	Yu, Liqin,et al."ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 33.6(2014):1354-1362.