Generation of Patient-Specific Induced Neuronal Cells Using a Direct Reprogramming Strategy

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	Generation of Patient-Specific Induced Neuronal Cells Using a Direct Reprogramming Strategy
	Wang, P ; Zhang, HL ; Li, WG ; Sha, HY ; Xu, CS ; Yao, L ; Tang, QS ; Tang, HL ; Chen, LP ; Zhu, JH
刊名	STEM CELLS AND DEVELOPMENT
	2014
卷号	23 期号:1 页码:16-23
关键词	PLURIPOTENT STEM-CELLS ADULT HUMAN FIBROBLASTS DIRECT CONVERSION DEFINED FACTORS FUNCTIONAL-NEURONS DOPAMINERGIC-NEURONS MOUSE FIBROBLASTS MOTOR-NEURONS IN-VIVO TRANSPLANTATION
ISSN号	1547-3287
通讯作者	Zhu, JH (reprint author), Fudan Univ, Shanghai Med Coll, Huashan Hosp, Natl Key Lab Med Neurobiol,Inst Brain Sci, Shanghai 200040, Peoples R China.,drjhzhu@gmail.com
英文摘要	Direct reprogramming of human fibroblasts into functional neurons in vitro by defined factors provides an invaluable resource for regenerative medicine. However, clinical applications must consider the risk of immune rejection, thus patient-specific induced neuronal cells (iNCs) may serve as an ideal source for autologous cell replacement. In this study, we report a robust process for functional neuronal cells from the patients' scalp by lentiviral gene delivery of Ascl1, Myt1l, and Sox2. These three-factor iNCs are similar to human neuronal cells in morphology, surface antigens, gene expression, and electrophysiological characteristics. Our findings might provide a source of patient-specific functional neurons for cell therapy.
学科主题	Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
收录类别	SCI
资助信息	National Nature Science Foundation [81271003, 2010CB945500, 2012CB966300, 2009CB941100, 11JC140100]; Ministry of Science and Technology of China; Shanghai City Science Foundation
语种	英语
公开日期	2014-07-30
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2635]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Wang, P,Zhang, HL,Li, WG,et al. Generation of Patient-Specific Induced Neuronal Cells Using a Direct Reprogramming Strategy[J]. STEM CELLS AND DEVELOPMENT,2014,23(1):16-23.
APA	Wang, P.,Zhang, HL.,Li, WG.,Sha, HY.,Xu, CS.,...&Zhu, JH.(2014).Generation of Patient-Specific Induced Neuronal Cells Using a Direct Reprogramming Strategy.STEM CELLS AND DEVELOPMENT,23(1),16-23.
MLA	Wang, P,et al."Generation of Patient-Specific Induced Neuronal Cells Using a Direct Reprogramming Strategy".STEM CELLS AND DEVELOPMENT 23.1(2014):16-23.