Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis

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	Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis
	Fang, Yan 2; Yang, Hongsheng1 ; Liu, Baozhong; Zhang, Libing ; Yang, HS
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
	2013
卷号	157 期号:1 页码:54-62
关键词	Clam Mollusk Lysozyme Metallothionein Superoxide dismutase Heavy metals Bacteria Mactra veneriformis
ISSN号	1532-0456
通讯作者	Yang, HS
中文摘要	The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved.
英文摘要	The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
关键词[WOS]	OYSTER CRASSOSTREA-VIRGINICA ; PACIFIC OYSTER ; MESSENGER-RNA ; MYTILUS-GALLOPROVINCIALIS ; MEDITERRANEAN MUSSEL ; DREISSENA-POLYMORPHA ; CERASTODERMA-EDULE ; CADMIUM EXPOSURE ; GENE-EXPRESSION ; CDNA CLONING
收录类别	SCI
原文出处	10.1016/j.cbpc.2012.10.002
语种	英语
WOS记录号	WOS:000312470600007
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16502]
专题	海洋研究所_海洋生态与环境科学重点实验室海洋研究所_实验海洋生物学重点实验室
通讯作者	Yang, HS
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Fang, Yan,Yang, Hongsheng,Liu, Baozhong,et al. Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2013,157(1):54-62.
APA	Fang, Yan,Yang, Hongsheng,Liu, Baozhong,Zhang, Libing,&Yang, HS.(2013).Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,157(1),54-62.
MLA	Fang, Yan,et al."Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 157.1(2013):54-62.