Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions
	Liu, Yun 1,2; Song, Xiuxian1 ; Cao, Xihua1 ; Yu, Zhiming1 ; Song, XX
刊名	JOURNAL OF PLANKTON RESEARCH
	2013
卷号	35 期号:1 页码:165-176
关键词	photosynthetic rate rbcL transcript levels nutrient concentrations quantitative real-time PCR Skeletonema costatum
ISSN号	0142-7873
通讯作者	Song, XX
中文摘要	The photosynthetic capacity of phytoplankton is frequently limited by the availability of nutrients. To explore the mechanism of the effect of nutrient availability on photosynthesis, batch cultures of a marine diatom Skeletonema costatum (Greville) Cleve were carried out under different nitrate (N: 0-220.75 mu M) and phosphate (P: 0-9.075 mu M) concentrations. Changes of photosynthetic characters, including the photosynthetic rate (measured by O-2 evolution) and the rbcL mRNA content (encoding the large subunit of the Calvin cycle enzyme, RuBisCO), were both studied. Within the range tested, both the photosynthetic rate and rbcL transcript levels correlated significantly with N and P concentrations in the medium. Additionally, the photosynthetic rates and rbcL transcript levels were both growth dependent. Significant correlations were also found between the abundance of rbcL mRNA and the photosynthetic rate (R-2 = 0.800), rbcL and growth rate (R-2 = 0.855), the photosynthetic rate and growth rate (R-2 = 0.815). These results are consistent with N and P nutrition-regulating photosynthesis from the transcriptional levels of rbcL. The rbcL transcript level has the potential to be a good marker of algal growth and primary productivity.
英文摘要	The photosynthetic capacity of phytoplankton is frequently limited by the availability of nutrients. To explore the mechanism of the effect of nutrient availability on photosynthesis, batch cultures of a marine diatom Skeletonema costatum (Greville) Cleve were carried out under different nitrate (N: 0-220.75 mu M) and phosphate (P: 0-9.075 mu M) concentrations. Changes of photosynthetic characters, including the photosynthetic rate (measured by O-2 evolution) and the rbcL mRNA content (encoding the large subunit of the Calvin cycle enzyme, RuBisCO), were both studied. Within the range tested, both the photosynthetic rate and rbcL transcript levels correlated significantly with N and P concentrations in the medium. Additionally, the photosynthetic rates and rbcL transcript levels were both growth dependent. Significant correlations were also found between the abundance of rbcL mRNA and the photosynthetic rate (R-2 = 0.800), rbcL and growth rate (R-2 = 0.855), the photosynthetic rate and growth rate (R-2 = 0.815). These results are consistent with N and P nutrition-regulating photosynthesis from the transcriptional levels of rbcL. The rbcL transcript level has the potential to be a good marker of algal growth and primary productivity.
学科主题	Marine & Freshwater Biology ; Oceanography
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Marine & Freshwater Biology ; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE ; ISOCHRYSIS-GALBANA HAPTOPHYCEAE ; ULVA-RIGIDA CHLOROPHYTA ; NITROGEN LIMITATION ; PHOSPHORUS LIMITATION ; INORGANIC-PHOSPHATE ; GENE-EXPRESSION ; BIOCHEMICAL-COMPOSITION ; CHLOROPLAST PROTEINS ; RIVER ESTUARY
收录类别	SCI
原文出处	10.1093/plankt/fbs080
语种	英语
WOS记录号	WOS:000312883300015
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16489]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Song, XX
作者单位	1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yun,Song, Xiuxian,Cao, Xihua,et al. Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions[J]. JOURNAL OF PLANKTON RESEARCH,2013,35(1):165-176.
APA	Liu, Yun,Song, Xiuxian,Cao, Xihua,Yu, Zhiming,&Song, XX.(2013).Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions.JOURNAL OF PLANKTON RESEARCH,35(1),165-176.
MLA	Liu, Yun,et al."Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions".JOURNAL OF PLANKTON RESEARCH 35.1(2013):165-176.