SmCCL19, a CC chemokine of turbot Scophthalmus maximus, induces leukocyte trafficking and promotes anti-viral and anti-bacterial defense

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	SmCCL19, a CC chemokine of turbot Scophthalmus maximus, induces leukocyte trafficking and promotes anti-viral and anti-bacterial defense
	Chen, Cheng 1,2; Hu, Yong-hua 1; Xiao, Zhi-zhong 1; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-11-01
卷号	35 期号:5 页码:1677-1682
关键词	Chemokine Scophthalmus maximus Chemotaxis Immune defense
ISSN号	1050-4648
通讯作者	Sun, L
中文摘要	Chemokines are classified into several different subfamilies, of which CC chemokines constitute the largest subfamily in teleost The prominent structural characteristic of CC chemokines is the presence of an Asp-Cys-Cys-Leu (DCCL) motif. To date, cDNA sequences of several CC chemokines have been identified in turbot (Scophthalmus maximus), however, the activity and function of these putative chemokines remain unknown. In this study, we examined the biological effect of the turbot CC chemokine SmCCL19, which has been previously reported as KC70 and shown to be regulated in expression by bacterial infection. To facilitate functional analysis, recombinant SmCCL19 (rSmCCL19) and a mutant form of SmCCL19, SmCCL19M, that bears serine substitutions at the two cysteine residues of the DCCL motif were purified from Escherichia coil. Chemotactic analysis showed that rSmCCL19 induced migration of head kidney leukocytes in a dose-dependent manner, whereas rSmCCL19M caused no apparent cellular migration. To examine the in vivo effect of rSmCCL19, turbot were administered with rSmCCL19 or rSmCCL19M before being inoculated with viral and bacterial pathogens. Subsequent tissue infection analysis showed that the viral and bacterial loads in rSmCCL19-adminsitered fish were significantly reduced, whereas the pathogen loads in rSmCCL19M-adminsitered fish were largely comparable to those in the control fish. Consistent with these observations, significant inductions of immune relevant genes were observed in rSmCCL19-adminsitered fish but not in rSmCCL19M-adminsitered fish. Taken together, these results indicate that SmCCL19 recruits leukocytes and augments host immune defense in a manner that depends on the conserved DCCL motif. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要	Chemokines are classified into several different subfamilies, of which CC chemokines constitute the largest subfamily in teleost The prominent structural characteristic of CC chemokines is the presence of an Asp-Cys-Cys-Leu (DCCL) motif. To date, cDNA sequences of several CC chemokines have been identified in turbot (Scophthalmus maximus), however, the activity and function of these putative chemokines remain unknown. In this study, we examined the biological effect of the turbot CC chemokine SmCCL19, which has been previously reported as KC70 and shown to be regulated in expression by bacterial infection. To facilitate functional analysis, recombinant SmCCL19 (rSmCCL19) and a mutant form of SmCCL19, SmCCL19M, that bears serine substitutions at the two cysteine residues of the DCCL motif were purified from Escherichia coil. Chemotactic analysis showed that rSmCCL19 induced migration of head kidney leukocytes in a dose-dependent manner, whereas rSmCCL19M caused no apparent cellular migration. To examine the in vivo effect of rSmCCL19, turbot were administered with rSmCCL19 or rSmCCL19M before being inoculated with viral and bacterial pathogens. Subsequent tissue infection analysis showed that the viral and bacterial loads in rSmCCL19-adminsitered fish were significantly reduced, whereas the pathogen loads in rSmCCL19M-adminsitered fish were largely comparable to those in the control fish. Consistent with these observations, significant inductions of immune relevant genes were observed in rSmCCL19-adminsitered fish but not in rSmCCL19M-adminsitered fish. Taken together, these results indicate that SmCCL19 recruits leukocytes and augments host immune defense in a manner that depends on the conserved DCCL motif. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	TROUT ONCORHYNCHUS-MYKISS ; CROAKER PSEUDOSCIAENA-CROCEA ; EXPRESSION ANALYSIS ; FUNCTIONAL-CHARACTERIZATION ; MOLECULAR CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; BACTERIAL-INFECTION ; JAPANESE FLOUNDER ; TELEOST FISH ; HOST-DEFENSE
收录类别	SCI
原文出处	10.1016/j.fsi.2013.08.020
语种	英语
WOS记录号	WOS:000328006700039
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16634]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Cheng,Hu, Yong-hua,Xiao, Zhi-zhong,et al. SmCCL19, a CC chemokine of turbot Scophthalmus maximus, induces leukocyte trafficking and promotes anti-viral and anti-bacterial defense[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(5):1677-1682.
APA	Chen, Cheng,Hu, Yong-hua,Xiao, Zhi-zhong,&Sun, Li.(2013).SmCCL19, a CC chemokine of turbot Scophthalmus maximus, induces leukocyte trafficking and promotes anti-viral and anti-bacterial defense.FISH & SHELLFISH IMMUNOLOGY,35(5),1677-1682.
MLA	Chen, Cheng,et al."SmCCL19, a CC chemokine of turbot Scophthalmus maximus, induces leukocyte trafficking and promotes anti-viral and anti-bacterial defense".FISH & SHELLFISH IMMUNOLOGY 35.5(2013):1677-1682.