Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications

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	Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications
	Lin, A-Peng 1; Wang, Guang-Ce 1; Zhou, Wen-Qin 2
刊名	PHYSIOLOGIA PLANTARUM
	2013-08-01
卷号	148 期号:4 页码:582-589
ISSN号	0031-9317
通讯作者	Wang, GC
中文摘要	Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an acid zone' in the apoplastic space (unstirred layer) of its leaves. It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O-2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall. To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted. In this study, experiments were undertaken on Z. marina by monitoring H+ and O-2 fluxes and the relative electron transport rates during light-dark transition. During stable photosynthesis, in addition to an obvious O-2 outflow, there was a significant net H+ influx connected to Z. marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O-2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.
英文摘要	Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an acid zone' in the apoplastic space (unstirred layer) of its leaves. It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O-2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall. To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted. In this study, experiments were undertaken on Z. marina by monitoring H+ and O-2 fluxes and the relative electron transport rates during light-dark transition. During stable photosynthesis, in addition to an obvious O-2 outflow, there was a significant net H+ influx connected to Z. marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O-2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.
学科主题	Plant Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	ION-SELECTIVE MICROELECTRODES ; INORGANIC CARBON UTILIZATION ; CHARA-CORALLINA ; PHOTOSYNTHETIC UTILIZATION ; PLASMA-MEMBRANE ; PROTON FLUXES ; SINGLE-CELL ; BICARBONATE ; SEAGRASSES ; TRANSPORT
收录类别	SCI
原文出处	10.1111/ppl.12008
语种	英语
WOS记录号	WOS:000321697300012
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16633]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Lin, A-Peng,Wang, Guang-Ce,Zhou, Wen-Qin. Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications[J]. PHYSIOLOGIA PLANTARUM,2013,148(4):582-589.
APA	Lin, A-Peng,Wang, Guang-Ce,&Zhou, Wen-Qin.(2013).Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications.PHYSIOLOGIA PLANTARUM,148(4),582-589.
MLA	Lin, A-Peng,et al."Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications".PHYSIOLOGIA PLANTARUM 148.4(2013):582-589.